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UPF3B Mouse mAb

#6349

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Western blotting analysis using UPF3B antibody (Cat#6349). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UPF3B antibody (Cat#6349, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with UPF3B antibody (Cat#6349, 1:1,000). Nuclei were stained blue with DAPI; UPF3B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of UPF3B expression in HeLa cells using UPF3B antibody (Cat#6349, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, UPF3B.
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Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    UPF3B
  • Catalog:
    6349
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H
Information
Product Name UPF3B Mouse mAb
Aliases UPF3B; UPF3B Regulator Of Nonsense Mediated MRNA Decay; RENT3B; UPF3X; HUPF3B; MRX82; Up-Frameshift Suppressor 3 Homolog On Chromosome X; Regulator Of Nonsense Transcripts 3B; Up-Frameshift Suppressor 3 Homolog B; Nonsense MRNA Reducing Factor 3B; UPF3BP1; UPF3BP2; UPF3BP3; MRX62; UPF3 Regulator Of Nonsense Transcripts Homolog B (Yeast); UPF3B, Regulator Of Nonsense Mediated MRNA Decay; UPF3 Regulator Of Nonsense Transcripts Homolog B; Mental Retardation, X-Linked 62; UPF3B Pseudogene 1; UPF3B Pseudogene 2; UPF3B Pseudogene 3; HUpf3p-X; Upf3p-X; MRXS14; HUpf3B
Background

Gene Name: UPF3B

NCBI Gene Entry: 65109

UniProt Entry: Q9BZI7

Application Information

Molecular Weight: Predicted, 58 kDa; observed, 62 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB8625

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen Recombinant protein of human UPF3B
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using UPF3B antibody (Cat#6349). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UPF3B antibody (Cat#6349, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of Hela cells with UPF3B antibody (Cat#6349, 1:1,000). Nuclei were stained blue with DAPI; UPF3B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of UPF3B expression in HeLa cells using UPF3B antibody (Cat#6349, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, UPF3B.
Western blotting analysis using UPF3B antibody (Cat#6349). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UPF3B antibody (Cat#6349, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with UPF3B antibody (Cat#6349, 1:1,000). Nuclei were stained blue with DAPI; UPF3B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of UPF3B expression in HeLa cells using UPF3B antibody (Cat#6349, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, UPF3B.
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