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Home>Product>Primary Antibody

KD-Validated HADHA Mouse mAb

#63587

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shRNA Knockdown Validated
Western blotting analysis using HADHA antibody (Cat#63587). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HADHA antibody (Cat#63587, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using HADHA antibody (Cat#63587). HADHA expression in wild type (WT) and HADHA shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with HADHA antibody (Cat#63587, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using HADHA antibody (Cat#63587, 1:1,000), Top panel: wild-type (WT); Bottom panal: HADHA shRNA knockdown (KD). Nuclei were stained blue with DAPI; HADHA was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HADHA expression in HepG2 cells using HADHA antibody (Cat#63587, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HADHA.
Validation of HADHA knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HADHA antibody (Cat#63587, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HADHA
  • Catalog：
    63587
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated HADHA Mouse mAb
Aliases HADHA; Hydroxyacyl-CoA Dehydrogenase Trifunctional Multienzyme Complex Subunit Alpha; LCHAD; LCEH; MTPA; GBP; Hydroxyacyl-Coenzyme A Dehydrogenase/3-Ketoacyl-Coenzyme A Thiolase/Enoyl-Coenzyme A Hydratase (Trifunctional Protein), Alpha Subunit; Hydroxyacyl-CoA Dehydrogenase/3-Ketoacyl-CoA Thiolase/Enoyl-CoA Hydratase (Trifunctional Protein), Alpha Subunit; Mitochondrial Trifunctional Protein, Alpha Subunit; Trifunctional Enzyme Subunit Alpha, Mitochondrial; Long-Chain-3-Hydroxyacyl-CoA Dehydrogenase; Monolysocardiolipin Acyltransferase; Long-Chain 2-Enoyl-CoA Hydratase; 78 KDa Gastrin-Binding Protein; Gastrin-Binding Protein; HADH; Mitochondrial Long-Chain L-3-Hydroxyacyl-Coenzyme A (CoA) Dehydrogenase, Alpha Subunit; Mitochondrial Long-Chain 2-Enoyl-Coenzyme A (CoA) Hydratase, Alpha Subunit; 3-Ketoacyl-Coenzyme A (CoA) Thiolase, Alpha Subunit; Mitochondrial Trifunctional Enzyme, Alpha Subunit; 3-Oxoacyl-CoA Thiolase; EC 2.3.1.- ; TP-ALPHA; TP-Alpha; ECHA
Background

Gene Name: HADHA

NCBI Gene Entry: 3030

UniProt Entry: P40939

Application Information

Molecular Weight: Predicted, 83 kDa; observed, 73 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB6655

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human HADHA
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using HADHA antibody (Cat#63587). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HADHA antibody (Cat#63587, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using HADHA antibody (Cat#63587). HADHA expression in wild type (WT) and HADHA shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with HADHA antibody (Cat#63587, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HeLa cells using HADHA antibody (Cat#63587, 1:1,000), Top panel: wild-type (WT); Bottom panal: HADHA shRNA knockdown (KD). Nuclei were stained blue with DAPI; HADHA was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of HADHA expression in HepG2 cells using HADHA antibody (Cat#63587, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HADHA.
  • Validation of HADHA knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HADHA antibody (Cat#63587, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    HADHA
  • Catalog：
    63587
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using HADHA antibody (Cat#63587). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HADHA antibody (Cat#63587, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using HADHA antibody (Cat#63587). HADHA expression in wild type (WT) and HADHA shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with HADHA antibody (Cat#63587, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using HADHA antibody (Cat#63587, 1:1,000), Top panel: wild-type (WT); Bottom panal: HADHA shRNA knockdown (KD). Nuclei were stained blue with DAPI; HADHA was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of HADHA expression in HepG2 cells using HADHA antibody (Cat#63587, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, HADHA.
Validation of HADHA knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with HADHA antibody (Cat#63587, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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