Western blotting analysis using heterogeneous nuclear ribonucleoprotein F antibody (Cat#63635). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with heterogeneous nuclear ribonucleoprotein F antibody (Cat#63635, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using heterogeneous nuclear ribonucleoprotein F antibody (Cat#63635). Heterogeneous nuclear ribonucleoprotein F expression in wild type (WT) and heterogeneous nuclear ribonucleoprotein F (HNRNPF) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with heterogeneous nuclear ribonucleoprotein F antibody (Cat#63635, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Heterogeneous nuclear ribonucleoprotein F antibody (Cat#63635, 1:1,000). Nuclei were stained blue with DAPI; Heterogeneous nuclear ribonucleoprotein F was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Heterogeneous nuclear ribonucleoprotein F expression in HepG2 cells using Heterogeneous nuclear ribonucleoprotein F antibody (Cat#63635, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Heterogeneous nuclear ribonucleoprotein F.
