KD-Validated CNP Mouse mAb - Product - Hefei GenuIN Biotech Co., Ltd.

KD-Validated CNP Mouse mAb

#63774

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shRNA Knockdown Validated

Western blotting analysis using CNP antibody (Cat#63774). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CNP antibody (Cat#63774, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using CNP antibody (Cat#63774). CNP expression in wild-type (WT) and CNP shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CNP antibody (Cat#63774, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Flow cytometric analysis of CNP expression in HepG2 cells using CNP antibody (Cat#63774, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CNP.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

CNP
Catalog：

63774
Application：

WB, FCM
Species Reactivity：

H

Information

Data

Information

Product Name	KD-Validated CNP Mouse mAb
Aliases	CNP; 2',3'-Cyclic Nucleotide 3' Phosphodiesterase; 2',3'-Cyclic-Nucleotide 3'-Phosphodiesterase; EC 3.1.4.37; CNPase; 2', 3' Cyclic Nucleotide 3' Phosphohydrolase; HLD20; CNP1
Background	Gene Name: CNP NCBI Gene Entry: 1267 UniProt Entry: P09543
Application Information	Molecular Weight: Predicted, 48 kDa; observed, 40 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB8575 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	Recombinant protein of human CNPase
Isotype	Mouse IgG1 Kappa
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using CNP antibody (Cat#63774). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CNP antibody (Cat#63774, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CNP antibody (Cat#63774). CNP expression in wild-type (WT) and CNP shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CNP antibody (Cat#63774, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of CNP expression in HepG2 cells using CNP antibody (Cat#63774, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, CNP.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

CNP
Catalog：

63774
Application：

WB, FCM
Species Reactivity：

H

Datasheet

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