Western blotting analysis using dipeptidyl peptidase 8 antibody (Cat#63853). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dipeptidyl peptidase 8 antibody (Cat#63853, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using dipeptidyl peptidase 8 antibody (Cat#63853). Dipeptidyl peptidase 8 expression in wild type (WT) and dipeptidyl peptidase 8 (DPP8) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with dipeptidyl peptidase 8 antibody (Cat#63853, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Dipeptidyl peptidase 8 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Dipeptidyl peptidase 8 antibody (Cat#63853, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
