Western blotting analysis using exosome component 1 antibody (Cat#63872). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with exosome component 1 antibody (Cat#63872, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using exosome component 1 antibody (Cat#63872). Exosome component 1 expression in wild-type (WT) and exosome component 1 (EXOSC1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with exosome component 1 antibody (Cat#63872, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with Exosome component 1 antibody (Cat#63872, 1:500). Nuclei were stained blue with DAPI; Exosome component 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using Exosome component 1 antibody (Cat#63872, 1:1,000), Top panel: wild-type (WT); Bottom panal: Exosome component 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Exosome component 1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of Exosome component 1 expression in HepG2 cells using Exosome component 1 antibody (Cat#63872, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Exosome component 1.
