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Home>Product>Primary Antibody

KD-Validated NAE1 Mouse mAb

#64857

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shRNA Knockdown Validated
Western blotting analysis using NAE1 antibody (Cat#64857). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NAE1 antibody (Cat#64857, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NAE1 antibody (Cat#64857). NAE1 expression in wild-type (WT) and NAE1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NAE1 antibody (Cat#64857, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using NAE1 antibody (Cat#64857, 1:1,000), Top panel: wild-type (WT); Bottom panal: NAE1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; NAE1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NAE1
  • Catalog：
    64857
  • Application：
    WB, ICC
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name KD-Validated NAE1 Mouse mAb
Aliases NAE1; NEDD8 Activating Enzyme E1 Subunit 1; APP-BP1; APPBP1; Ula-1; Amyloid Beta Precursor Protein-Binding Protein 1, 59 KDa ; Amyloid Beta Precursor Protein Binding Protein 1, 59kDa; NEDD8-Activating Enzyme E1 Regulatory Subunit; Amyloid Protein-Binding Protein 1; Proto-Oncogene Protein 1; Amyloid Beta Precursor Protein-Binding Protein 1, 59kD; NEDD8-Activating Enzyme E1 Subunit; Protooncogene Protein 1; A-116A10.1; NEDFIH; HPP1
Background

Gene Name: NAE1

NCBI Gene Entry: 8883

UniProt Entry: Q13564

Application Information

Molecular Weight: Predicted, 60 kDa; observed, 60 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB13290

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human NAE1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using NAE1 antibody (Cat#64857). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NAE1 antibody (Cat#64857, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using NAE1 antibody (Cat#64857). NAE1 expression in wild-type (WT) and NAE1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NAE1 antibody (Cat#64857, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HeLa cells using NAE1 antibody (Cat#64857, 1:1,000), Top panel: wild-type (WT); Bottom panal: NAE1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; NAE1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NAE1
  • Catalog：
    64857
  • Application：
    WB, ICC
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using NAE1 antibody (Cat#64857). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NAE1 antibody (Cat#64857, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NAE1 antibody (Cat#64857). NAE1 expression in wild-type (WT) and NAE1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NAE1 antibody (Cat#64857, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using NAE1 antibody (Cat#64857, 1:1,000), Top panel: wild-type (WT); Bottom panal: NAE1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; NAE1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
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