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Home>Product>Primary Antibody

KD-Validated SPTAN1 Mouse mAb

#64875

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shRNA Knockdown Validated
Western blotting analysis using SPTAN1 antibody (Cat#64875). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SPTAN1 antibody (Cat#64875, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using SPTAN1 antibody (Cat#64875). SPTAN1 expression in wild-type (WT) and SPTAN1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SPTAN1 antibody (Cat#64875, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using SPTAN1 antibody (Cat#64875, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of C2C12 cells with SPTAN1 antibody (Cat#64875, 1:1,000). Nuclei were stained blue with DAPI; SPTAN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using SPTAN1 antibody (Cat#64875, 1:1,000), Top panel: wild-type (WT); Bottom panal: SPTAN1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SPTAN1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Validation of SPTAN1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with SPTAN1 antibody (Cat#64875, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SPTAN1
  • Catalog：
    64875
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated SPTAN1 Mouse mAb
Aliases SPTAN1; Spectrin Alpha, Non-Erythrocytic 1; Spectrin Alpha Chain, Non-Erythrocytic 1; Spectrin, Non-Erythroid Alpha Subunit; Fodrin Alpha Chain; Alpha-II Spectrin; Alpha-Fodrin; SPTA2; Epididymis Secretory Sperm Binding Protein; Spectrin, Non-Erythroid Alpha Chain; HMND11; DEVEP; EIEE5; HMN11; SPG91; DEE5
Background

Gene Name: SPTAN1

NCBI Gene Entry: 6709

UniProt Entry: Q13813

Application Information

Molecular Weight: Predicted, 285 kDa; observed, 285 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB13380

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen Recombinant protein of human SPTAN1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:500-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using SPTAN1 antibody (Cat#64875). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SPTAN1 antibody (Cat#64875, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using SPTAN1 antibody (Cat#64875). SPTAN1 expression in wild-type (WT) and SPTAN1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SPTAN1 antibody (Cat#64875, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using SPTAN1 antibody (Cat#64875, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of C2C12 cells with SPTAN1 antibody (Cat#64875, 1:1,000). Nuclei were stained blue with DAPI; SPTAN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Immunocytochemical staining of HeLa cells using SPTAN1 antibody (Cat#64875, 1:1,000), Top panel: wild-type (WT); Bottom panal: SPTAN1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SPTAN1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Validation of SPTAN1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with SPTAN1 antibody (Cat#64875, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    SPTAN1
  • Catalog：
    64875
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using SPTAN1 antibody (Cat#64875). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SPTAN1 antibody (Cat#64875, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using SPTAN1 antibody (Cat#64875). SPTAN1 expression in wild-type (WT) and SPTAN1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SPTAN1 antibody (Cat#64875, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human hepatocarcinoma using SPTAN1 antibody (Cat#64875, 1:1,000). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of C2C12 cells with SPTAN1 antibody (Cat#64875, 1:1,000). Nuclei were stained blue with DAPI; SPTAN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using SPTAN1 antibody (Cat#64875, 1:1,000), Top panel: wild-type (WT); Bottom panal: SPTAN1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SPTAN1 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Validation of SPTAN1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with SPTAN1 antibody (Cat#64875, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use.
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