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Home>Product>Primary Antibody

KD-Validated NEDD4 Mouse mAb

#64880

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shRNA Knockdown Validated
Western blotting analysis using NEDD4 antibody (Cat#64880). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NEDD4 antibody (Cat#64880, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NEDD4 antibody (Cat#64880). NEDD4 expression in wild-type (WT) and NEDD4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with NEDD4 antibody (Cat#64880, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NEDD4
  • Catalog：
    64880
  • Application：
    WB
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated NEDD4 Mouse mAb
Aliases NEDD4; NEDD4 E3 Ubiquitin Protein Ligase; NEDD4-1; RPF1; KIAA0093; Neural Precursor Cell Expressed, Developmentally Down-Regulated 4, E3 Ubiquitin Protein Ligase; Cell Proliferation-Inducing Gene 53 Protein; HECT-Type E3 Ubiquitin Transferase NEDD4; E3 Ubiquitin-Protein Ligase NEDD4; Receptor-Potentiating Factor 1; MGC176705; Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 4; Neural Precursor Cell Expressed, Developmentally Down-Regulated 4; EC 2.3.2.26; EC 6.3.2; NEDD-4
Background

Gene Name: NEDD4

NCBI Gene Entry: 4734

UniProt Entry: P46934

Application Information

Molecular Weight: Predicted, 149 kDa; observed, 130 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB15775

Species Reactivity: Human

Applications Tested: Western blotting (WB)
Immunogen Recombinant protein of human NEDD4
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Note This product is for research use only.
Data
  • Western blotting analysis using NEDD4 antibody (Cat#64880). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NEDD4 antibody (Cat#64880, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using NEDD4 antibody (Cat#64880). NEDD4 expression in wild-type (WT) and NEDD4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with NEDD4 antibody (Cat#64880, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NEDD4
  • Catalog：
    64880
  • Application：
    WB
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using NEDD4 antibody (Cat#64880). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NEDD4 antibody (Cat#64880, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NEDD4 antibody (Cat#64880). NEDD4 expression in wild-type (WT) and NEDD4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with NEDD4 antibody (Cat#64880, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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