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Home>Product>Primary Antibody

KD-Validated MAP2K1 Rabbit pAb

#64967

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shRNA Knockdown Validated
Western blotting analysis using MAP2K1 antibody (Cat#64967). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAP2K1 antibody (Cat#64967, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using MAP2K1 antibody (Cat #64967). MAP2K1 expression in wild-type (WT) and MAP2K1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAP2K1 antibody (Cat #64967, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MAP2K1
  • Catalog：
    64967
  • Application：
    WB
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated MAP2K1 Rabbit pAb
Aliases MAP2K1; Mitogen-Activated Protein Kinase Kinase 1; MEK1; MKK1; Dual Specificity Mitogen-Activated Protein Kinase Kinase 1; MAPK/ERK Kinase 1; MAPKK1; PRKMK1; ERK Activator Kinase 1; MAP Kinase Kinase 1; EC 2.7.12.2; MAPKK 1; MEK 1; Protein Kinase, Mitogen-Activated, Kinase 1 (MAP Kinase Kinase 1); CFC3; MEL
Background

Gene Name: MAP2K1

NCBI Gene Entry: 5604

UniProt Entry: Q02750

Application Information

Molecular Weight: Predicted, 43 kDa; observed, 45 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human MKK1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Note This product is for research use only.
Data
  • Western blotting analysis using MAP2K1 antibody (Cat#64967). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAP2K1 antibody (Cat#64967, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using MAP2K1 antibody (Cat #64967). MAP2K1 expression in wild-type (WT) and MAP2K1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAP2K1 antibody (Cat #64967, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    MAP2K1
  • Catalog：
    64967
  • Application：
    WB
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using MAP2K1 antibody (Cat#64967). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAP2K1 antibody (Cat#64967, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using MAP2K1 antibody (Cat #64967). MAP2K1 expression in wild-type (WT) and MAP2K1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAP2K1 antibody (Cat #64967, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
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