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KD-Validated PRKCD Mouse mAb

#62153

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shRNA Knockdown Validated
Western blotting analysis using PRKCD antibody (Cat#62153). PRKCD expression in wild-type (WT) and PRKCD shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PRKCD antibody (Cat#62153, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PRKCD
  • Catalog:
    62153
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated PRKCD Mouse mAb
Aliases PRKCD; Protein Kinase C Delta; Tyrosine-Protein Kinase PRKCD; Protein Kinase C Delta Type; EC 2.7.11.13; NPKC-Delta; PKCD; Protein Kinase C Delta VIII; Protein Kinase C, Delta; EC 2.7.10.2; EC 2.7.11; ALPS3; CVID9; MAY1
Background

Gene Name: PRKCD

NCBI Gene Entry: 5580

UniProt Entry: Q05655

Application Information

Molecular Weight: Predicted, 78 kDa; observed, 70 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB1615

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human PRKCD
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using PRKCD antibody (Cat#62153). PRKCD expression in wild-type (WT) and PRKCD shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PRKCD antibody (Cat#62153, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PRKCD antibody (Cat#62153). PRKCD expression in wild-type (WT) and PRKCD shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PRKCD antibody (Cat#62153, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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