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Home>Product>Primary Antibody

GPA33 Recombinant Rabbit mAb

#H1134

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Western blotting analysis using glycoprotein A33 antibody (Cat#H1134). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with glycoprotein A33 antibody (Cat#H1134, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Immunocytochemical staining shows negative staining in paraffin-embedded human breast cancer. GPA33 antibody (Cat#H1134) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human skeletal muscle. GPA33 antibody (Cat#H1134) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human kidney. GPA33 antibody (Cat#H1134) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human colon cancer. GPA33 antibody (Cat#H1134) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human colon. GPA33 antibody (Cat#H1134) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    GPA33
  • Catalog：
    H1134
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name GPA33 Recombinant Rabbit mAb
Aliases GPA33; Glycoprotein A33; A33; Glycoprotein A33 (Transmembrane); Cell Surface A33 Antigen; Transmembrane Glycoprotein A33
Background

Gene Name: GPA33

NCBI Gene Entry: 10223

UniProt Entry: Q99795

Application Information

Molecular Weight: Predicted, 36 kDa; observed, 48 kDa

Clonality: Recombinant Monoclonal Antibody

Clone ID: SDT-248-76

Species Reactivity: Human ,rat

Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human GPA33
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 40% glycerol, 0.05% BSA and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunohistochemistry-Paraffin (IHC-P): 1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using glycoprotein A33 antibody (Cat#H1134). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with glycoprotein A33 antibody (Cat#H1134, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining shows negative staining in paraffin-embedded human breast cancer. GPA33 antibody (Cat#H1134) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows negative staining in paraffin-embedded human skeletal muscle. GPA33 antibody (Cat#H1134) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows negative staining in paraffin-embedded human kidney. GPA33 antibody (Cat#H1134) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human colon cancer. GPA33 antibody (Cat#H1134) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
  • Immunocytochemical staining shows positive staining in paraffin-embedded human colon. GPA33 antibody (Cat#H1134) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    GPA33
  • Catalog：
    H1134
  • Application：
    WB, IHC-P
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using glycoprotein A33 antibody (Cat#H1134). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with glycoprotein A33 antibody (Cat#H1134, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Immunocytochemical staining shows negative staining in paraffin-embedded human breast cancer. GPA33 antibody (Cat#H1134) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human skeletal muscle. GPA33 antibody (Cat#H1134) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows negative staining in paraffin-embedded human kidney. GPA33 antibody (Cat#H1134) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human colon cancer. GPA33 antibody (Cat#H1134) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
Immunocytochemical staining shows positive staining in paraffin-embedded human colon. GPA33 antibody (Cat#H1134) was used at 1:2,000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with Immunocytochemical staining protocol.
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