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  • Primary Antibody
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  • Lentivirus
  • Cell  Lines
  • Lysates
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APEX1 Mouse mAb

#6420

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Western blotting analysis using APEX1 antibody (Cat#6420). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with APEX1 antibody (Cat#6420, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with APEX1 antibody (Cat#6420, 1:1,000) . Nuclei were stained blue with DAPI; APEX1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of APEX1 expression in HeLa cells using APEX1 antibody (Cat#6420,1:2,000). Green, isotype control; red, APEX1.
Please select the specifications of the product.
Specification：
20 μL，100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    APEX1
  • Catalog：
    6420
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name APEX1 Mouse mAb
Aliases APEX1; Apurinic/Apyrimidinic Endodeoxyribonuclease 1; REF1; HAP1; APEN; APE; APX; REF-1; APE-1; APEX; APEX Nuclease (Multifunctional DNA Repair Enzyme) 1; DNA-(Apurinic Or Apyrimidinic Site) Endonuclease; DNA Repair Nuclease/Redox Regulator APEX1; Apurinic-Apyrimidinic Endonuclease 1; Redox Factor-1; APE1; APEX Nuclease (Multifunctional DNA Repair Enzyme); Deoxyribonuclease (Apurinic Or Apyrimidinic); Apurinic/Apyrimidinic (Abasic) Endonuclease; DNA-(Apurinic Or Apyrimidinic Site) Lyase; AP Endonuclease Class I; AP Endonuclease 1; Protein REF-1; APEX Nuclease; EC 4.2.99.18; EC 3.1.11.2; EC 3.1.21.-; AP Lyase
Background

Gene Name: APEX1

NCBI Gene Entry: 328

UniProt Entry: P27695

Application Information

Molecular Weight: Predicted, 36 kDa; observed, 35 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9010

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human APEX1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:4,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using APEX1 antibody (Cat#6420). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with APEX1 antibody (Cat#6420, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of Hela cells with APEX1 antibody (Cat#6420, 1:1,000) . Nuclei were stained blue with DAPI; APEX1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Flow cytometric analysis of APEX1 expression in HeLa cells using APEX1 antibody (Cat#6420,1:2,000). Green, isotype control; red, APEX1.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    APEX1
  • Catalog：
    6420
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using APEX1 antibody (Cat#6420). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with APEX1 antibody (Cat#6420, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with APEX1 antibody (Cat#6420, 1:1,000) . Nuclei were stained blue with DAPI; APEX1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of APEX1 expression in HeLa cells using APEX1 antibody (Cat#6420,1:2,000). Green, isotype control; red, APEX1.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
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  149. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
  154. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_cart.php ( 3.88 KB )
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  158. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-trace/src/Html.php ( 4.42 KB )
  1. CONNECT:[ UseTime:0.003531s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001849s ]
  3. SELECT * FROM `goods` WHERE `code` = '6420' LIMIT 1 [ RunTime:0.001187s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000894s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000973s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000748s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 10257 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001018s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.002648s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 10257 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000254s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000402s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.001948s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.059030s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (8953,4754,3963,4821,4173,4782,3765,6864,7238,5581) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000853s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000480s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000204s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000502s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000226s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000326s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000436s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000240s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000244s ]
0.114489s