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KD-Validated PARK7 Rabbit pAb

#64981

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shRNA Knockdown Validated
Western blotting analysis using PARK7 antibody (Cat#64981). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PARK7 antibody (Cat#64981, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PARK7 antibody (Cat #64981). PARK7 expression in wild-type (WT) and PARK7 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PARK7 antibody (Cat #64981, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
Please select the specifications of the product.
Specification:
Unit price:
$89.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    PARK7
  • Catalog:
    64981
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated PARK7 Rabbit pAb
Aliases PARK7; Parkinsonism Associated Deglycase; DJ-1; GATD2; DJ1; Parkinson Disease (Autosomal Recessive, Early Onset) 7; Protein/Nucleic Acid Deglycase DJ-1; Parkinson Disease Protein 7; Parkinson Protein 7; Maillard Deglycase; Oncogene DJ1; Protein DJ-1; Epididymis Secretory Sperm Binding Protein Li 67p; Parkinsonism-Associated Deglycase; Protein Deglycase DJ-1; EC 3.5.1.124; EC 3.1.2.-; EC 3.5.1.-; HEL-S-67p
Background

Gene Name: PARK7

NCBI Gene Entry: 11315

UniProt Entry: Q99497

Application Information

Molecular Weight: Predicted, 20 kDa; observed, 22 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human DJ-1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:200-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PARK7 antibody (Cat#64981). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PARK7 antibody (Cat#64981, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using PARK7 antibody (Cat #64981). PARK7 expression in wild-type (WT) and PARK7 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PARK7 antibody (Cat #64981, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
Western blotting analysis using PARK7 antibody (Cat#64981). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PARK7 antibody (Cat#64981, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PARK7 antibody (Cat #64981). PARK7 expression in wild-type (WT) and PARK7 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PARK7 antibody (Cat #64981, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
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