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TOR1AIP1 Mouse mAb

#6381

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Western blotting analysis using TOR1AIP1 antibody (Cat#6381). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TOR1AIP1 antibody (Cat#6381, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with TOR1AIP1 antibody (Cat#6381, 1:1,000). Nuclei were stained blue with DAPI; TOR1AIP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TOR1AIP1 expression in HepG2 cells using TOR1AIP1 antibody (Cat#6381, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TOR1AIP1.
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Unit price:
$69.00
Quantity:
1
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  • Gene Symbol:
    TOR1AIP1
  • Catalog:
    6381
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H
Information
Product Name TOR1AIP1 Mouse mAb
Aliases TOR1AIP1; Torsin 1A Interacting Protein 1; LAP1B; LAP1; LAP1C; Torsin-1A-Interacting Protein 1; Torsin A Interacting Protein 1; Lamin-Associated Protein 1B; FLJ13142; Lamina Associated Polypeptide 1B; Lamina-Associated Polypeptide 1B; LGMD2Y
Background

Gene Name: TOR1AIP1

NCBI Gene Entry: 26092

UniProt Entry: Q5JTV8

Application Information

Molecular Weight: Predicted, 66 kDa; observed, 55 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB8780

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen Recombinant protein of human TOR1AIP1
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using TOR1AIP1 antibody (Cat#6381). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TOR1AIP1 antibody (Cat#6381, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with TOR1AIP1 antibody (Cat#6381, 1:1,000). Nuclei were stained blue with DAPI; TOR1AIP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of TOR1AIP1 expression in HepG2 cells using TOR1AIP1 antibody (Cat#6381, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TOR1AIP1.
Western blotting analysis using TOR1AIP1 antibody (Cat#6381). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TOR1AIP1 antibody (Cat#6381, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with TOR1AIP1 antibody (Cat#6381, 1:1,000). Nuclei were stained blue with DAPI; TOR1AIP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TOR1AIP1 expression in HepG2 cells using TOR1AIP1 antibody (Cat#6381, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, TOR1AIP1.
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