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Home>Product>Primary Antibody

KD-Validated Cleaved-Caspase 8 Rabbit pAb

#65013

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shRNA Knockdown Validated
Western blotting analysis using caspase 8 antibody (Cat#65013). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 8 antibody (Cat#65013, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using caspase 8 antibody (Cat #65013). Caspase 8 expression in wild-type (WT) and caspase 8 (CASP8) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with caspase 8 antibody (Cat #65013, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CASP8
  • Catalog：
    65013
  • Application：
    WB
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated Cleaved-Caspase 8 Rabbit pAb
Aliases CASP8; Caspase 8; FLICE; MCH5; MACH; Casp-8; Caspase 8, Apoptosis-Related Cysteine Peptidase; Caspase 8, Apoptosis-Related Cysteine Protease; MORT1-Associated Ced-3 Homolog; ICE-Like Apoptotic Protease 5; Apoptotic Cysteine Protease; Apoptotic Protease Mch-5; FADD-Like ICE; Caspase-8; CAP4; FADD-Homologous ICE/CED-3-Like Protease; FADD-Homologous ICE/Ced-3-Like Protease; MACH-Beta-1/2/3/4 Protein; MACH-Alpha-1/2/3 Protein; EC 3.4.22.61; ALPS2B; CASP-8
Background

Gene Name: CASP8

NCBI Gene Entry: 841

UniProt Entry: Q14790

Application Information

Molecular Weight: Predicted, 55 kDa; observed, 28 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human Cleaved Caspase 8
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Note This product is for research use only.
Data
  • Western blotting analysis using caspase 8 antibody (Cat#65013). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 8 antibody (Cat#65013, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using caspase 8 antibody (Cat #65013). Caspase 8 expression in wild-type (WT) and caspase 8 (CASP8) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with caspase 8 antibody (Cat #65013, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    CASP8
  • Catalog：
    65013
  • Application：
    WB
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using caspase 8 antibody (Cat#65013). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with caspase 8 antibody (Cat#65013, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using caspase 8 antibody (Cat #65013). Caspase 8 expression in wild-type (WT) and caspase 8 (CASP8) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with caspase 8 antibody (Cat #65013, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
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