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Home>Product>Primary Antibody

KD-Validated PIK3C2A Mouse mAb

#65052

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shRNA Knockdown Validated
Western blotting analysis using PIK3C2A antibody (Cat#65052). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PIK3C2A antibody (Cat#65052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PIK3C2A antibody (Cat#65052). PIK3C2A expression in wild-type (WT) and PIK3C2A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PIK3C2A antibody (Cat#65052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with PIK3C2A antibody (Cat#65052, 1:1,000). Nuclei were stained blue with DAPI; PIK3C2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of PIK3C2A expression in HepG2 cells using PIK3C2A antibody (Cat#65052, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PIK3C2A.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PIK3C2A
  • Catalog：
    65052
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated PIK3C2A Mouse mAb
Aliases PIK3C2A; Phosphatidylinositol-4-Phosphate 3-Kinase Catalytic Subunit Type 2 Alpha; Phosphatidylinositol 4-Phosphate 3-Kinase C2 Domain-Containing Subunit Alpha; PI3K-C2alpha; Phosphoinositide-3-Kinase, Class 2, Alpha Polypeptide; Phosphoinositide 3-Kinase-C2-Alpha; PtdIns-3-Kinase C2 Subunit Alpha; PI3K-C2-Alpha; EC 2.7.1.154; Phosphatidylinositol-4-Phosphate 3-Kinase C2 Domain-Containing Subunit Alpha; Phosphatidylinositol-4-Phosphate 3-Kinase, Catalytic Subunit Type 2 Alpha; C2-Containing Phosphatidylinositol Kinase; PI3-K-C2(ALPHA); EC 2.7.1.137; EC 2.7.1.153; PI3-K-C2A; EC 2.7.1; OCSKD; CPK
Background

Gene Name: PIK3C2A

NCBI Gene Entry: 5286

UniProt Entry: O00443

Application Information

Molecular Weight: Predicted, 191 kDa; observed, 191 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB14050

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human PIK3C2A
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:100-1:1,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using PIK3C2A antibody (Cat#65052). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PIK3C2A antibody (Cat#65052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using PIK3C2A antibody (Cat#65052). PIK3C2A expression in wild-type (WT) and PIK3C2A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PIK3C2A antibody (Cat#65052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HepG2 cells with PIK3C2A antibody (Cat#65052, 1:1,000). Nuclei were stained blue with DAPI; PIK3C2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of PIK3C2A expression in HepG2 cells using PIK3C2A antibody (Cat#65052, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PIK3C2A.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PIK3C2A
  • Catalog：
    65052
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using PIK3C2A antibody (Cat#65052). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PIK3C2A antibody (Cat#65052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PIK3C2A antibody (Cat#65052). PIK3C2A expression in wild-type (WT) and PIK3C2A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PIK3C2A antibody (Cat#65052, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with PIK3C2A antibody (Cat#65052, 1:1,000). Nuclei were stained blue with DAPI; PIK3C2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of PIK3C2A expression in HepG2 cells using PIK3C2A antibody (Cat#65052, 1:1,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PIK3C2A.
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