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Home>Product>Primary Antibody

KD-Validated DAB2 Recombinant Rabbit mAb

#65221

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using DAB2 antibody (Cat#65221). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DAB2 antibody (Cat#65221, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using DAB2 antibody (Cat#65221). DAB2 expression in wild-type (WT) and DAB2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DAB2 antibody (Cat#65221, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with DAB2 antibody (Cat#65221, 1:1,000). Nuclei were stained blue with DAPI; DAB2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of DAB2 expression in HepG2 cells using DAB2 antibody (Cat#65221, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DAB2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DAB2
  • Catalog：
    65221
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated DAB2 Recombinant Rabbit mAb
Aliases DAB2; DAB Adaptor Protein 2; DOC-2; Differentially Expressed In Ovarian Carcinoma 2; Differentially-Expressed Protein 2; DAB2, Clathrin Adaptor Protein 2; Adaptor Molecule Disabled-2; Disabled Homolog 2; DOC2; Disabled (Drosophila) Homolog 2 (Mitogen-Responsive Phosphoprotein); Disabled Homolog 2, Mitogen-Responsive Phosphoprotein (Drosophila); Dab, Mitogen-Responsive Phosphoprotein, Homolog 2 (Drosophila); Disabled Homolog 2, Mitogen-Responsive Phosphoprotein; Dab, Mitogen-Responsive Phosphoprotein, Homolog 2
Background

Gene Name: DAB2

NCBI Gene Entry: 1601

UniProt Entry: P98082

Application Information

Molecular Weight: Predicted, 82 kDa; observed, 96 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB225

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human DAB2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using DAB2 antibody (Cat#65221). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DAB2 antibody (Cat#65221, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using DAB2 antibody (Cat#65221). DAB2 expression in wild-type (WT) and DAB2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DAB2 antibody (Cat#65221, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HepG2 cells with DAB2 antibody (Cat#65221, 1:1,000). Nuclei were stained blue with DAPI; DAB2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Flow cytometric analysis of DAB2 expression in HepG2 cells using DAB2 antibody (Cat#65221, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DAB2.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DAB2
  • Catalog：
    65221
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using DAB2 antibody (Cat#65221). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DAB2 antibody (Cat#65221, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using DAB2 antibody (Cat#65221). DAB2 expression in wild-type (WT) and DAB2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DAB2 antibody (Cat#65221, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with DAB2 antibody (Cat#65221, 1:1,000). Nuclei were stained blue with DAPI; DAB2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Flow cytometric analysis of DAB2 expression in HepG2 cells using DAB2 antibody (Cat#65221, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, DAB2.
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