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Home>Product>Primary Antibody

KD-Validated BCAM Recombinant Rabbit mAb

#65254

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using BCAM antibody (Cat#65254). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCAM antibody (Cat#65254, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using BCAM antibody (Cat#65254). BCAM expression in wild-type (WT) and BCAM shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BCAM antibody (Cat#65254, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    BCAM
  • Catalog：
    65254
  • Application：
    WB
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated BCAM Recombinant Rabbit mAb
Aliases BCAM; Basal Cell Adhesion Molecule (Lutheran Blood Group); F8/G253; CD239; B-CAM; LU; Basal Cell Adhesion Molecule (Lu And Au Blood Groups); B-CAM Cell Surface Glycoprotein; Basal Cell Adhesion Molecule; Auberger B Antigen; F8/G253 Antigen; MSK19; Lutheran Blood Group (Auberger B Antigen Included); Lutheran Blood Group Variant LUGA; Lutheran Blood Group Glycoprotein; B-Cell Adhesion Molecule; Lutheran Antigen; CD239 Antigen; AU
Background

Gene Name: BCAM

NCBI Gene Entry: 4059

UniProt Entry: P50895

Application Information

Molecular Weight: Predicted, 67 kDa; observed, 80 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB375

Species Reactivity: Human

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human BCAM
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using BCAM antibody (Cat#65254). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCAM antibody (Cat#65254, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using BCAM antibody (Cat#65254). BCAM expression in wild-type (WT) and BCAM shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BCAM antibody (Cat#65254, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    BCAM
  • Catalog：
    65254
  • Application：
    WB
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using BCAM antibody (Cat#65254). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCAM antibody (Cat#65254, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using BCAM antibody (Cat#65254). BCAM expression in wild-type (WT) and BCAM shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with BCAM antibody (Cat#65254, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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