KD-Validated MIB1 Recombinant Rabbit mAb - Product

KD-Validated MIB1 Recombinant Rabbit mAb

#65263

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Recombinant Antibody
shRNA Knockdown Validated

Western blotting analysis using MIB1 antibody (Cat#65263). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MIB1 antibody (Cat#65263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using MIB1 antibody (Cat#65263). MIB1 expression in wild-type (WT) and MIB1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MIB1 antibody (Cat#65263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Flow cytometric analysis of MIB1 expression in HepG2 cells using MIB1 antibody (Cat#65263, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, MIB1.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

MIB1
Catalog：

65263
Application：

WB, FCM
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KD-Validated MIB1 Recombinant Rabbit mAb
Aliases	MIB1; MIB E3 Ubiquitin Protein Ligase 1; ZZANK2; DIP-1; DAPK-Interacting Protein 1; KIAA1323; ZZZ6; MIB; Zinc Finger ZZ Type With Ankyrin Repeat Domain Protein; RING-Type E3 Ubiquitin Transferase MIB1; E3 Ubiquitin-Protein Ligase MIB1; DIP1; Mindbomb E3 Ubiquitin Protein Ligase 1; Mindbomb Homolog 1 (Drosophila); Ubiquitin Ligase Mind Bomb; Mind Bomb Homolog 1; EC 2.3.2.27; EC 6.3.2; LVNC7
Background	Gene Name: MIB1 NCBI Gene Entry: 57534 UniProt Entry: Q86YT6
Application Information	Molecular Weight: Predicted, 110 kDa; observed, 110 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB420 Species Reactivity: Human,mouse,rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human MIB1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using MIB1 antibody (Cat#65263). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MIB1 antibody (Cat#65263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using MIB1 antibody (Cat#65263). MIB1 expression in wild-type (WT) and MIB1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MIB1 antibody (Cat#65263, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of MIB1 expression in HepG2 cells using MIB1 antibody (Cat#65263, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, MIB1.

Please select the specifications of the product.

Specification：

10 μL 20 μL 100 μL

Unit price：

$89.00

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

MIB1
Catalog：

65263
Application：

WB, FCM
Species Reactivity：

H, M, R

Datasheet

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