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Home>Product>Primary Antibody

KD-Validated KPNA1 Mouse mAb

#65275

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Knockdown Validated
Western blotting analysis using KPNA1 antibody (Cat#65275). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KPNA1 antibody (Cat#65275, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using KPNA1 antibody (Cat#65275). KPNA1 expression in wild-type (WT) and KPNA1 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with KPNA1 antibody (Cat#65275, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of KPNA1 expression in HepG2 cells using KPNA1 antibody (Cat#65275, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, KPNA1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    KPNA1
  • Catalog：
    65275
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated KPNA1 Mouse mAb
Aliases KPNA1; Karyopherin Subunit Alpha 1; NPI-1; RCH2; IPOA5; SRP1; Karyopherin Alpha 1 (Importin Alpha 5); Nucleoprotein Interactor 1; Importin Subunit Alpha-5; RAG Cohort Protein 2; Importin Alpha 5; SRP1-Beta; Recombination Activating Gene Cohort; Karyopherin Subunit Alpha-1; Importin Subunit Alpha-1; Karyopherin Alpha 1; Importin-Alpha-S1
Background

Gene Name: KPNA1

NCBI Gene Entry: 3836

UniProt Entry: P52294

Application Information

Molecular Weight: Predicted, 60 kDa; observed, 62-65 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB700

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human KPNA1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using KPNA1 antibody (Cat#65275). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KPNA1 antibody (Cat#65275, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using KPNA1 antibody (Cat#65275). KPNA1 expression in wild-type (WT) and KPNA1 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with KPNA1 antibody (Cat#65275, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of KPNA1 expression in HepG2 cells using KPNA1 antibody (Cat#65275, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, KPNA1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    KPNA1
  • Catalog：
    65275
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using KPNA1 antibody (Cat#65275). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with KPNA1 antibody (Cat#65275, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using KPNA1 antibody (Cat#65275). KPNA1 expression in wild-type (WT) and KPNA1 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with KPNA1 antibody (Cat#65275, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of KPNA1 expression in HepG2 cells using KPNA1 antibody (Cat#65275, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, KPNA1.
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