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Tumor Protein P53 Mouse mAb

#6528

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Western blotting analysis using tumor protein p53 antibody (Cat#6528). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with tumor protein p53 antibody (Cat#6528, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of Hela cells with tumor protein p53 antibody (Cat#6528, 1:1,000). Nuclei were stained blue with DAPI; Tumor protein p53 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TP53 expression in HeLa cells using TP53 antibody (Cat#6528, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TP53.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    TP53
  • Catalog:
    6528
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name Tumor Protein P53 Mouse mAb
Aliases TP53; Tumor Protein P53; P53; LFS1; Cellular Tumor Antigen P53; Phosphoprotein P53; Antigen NY-CO-13; Transformation-Related Protein 53; Mutant Tumor Protein 53; Li-Fraumeni Syndrome; Tumor Suppressor P53; Tumor Supressor P53; Tumor Protein 53; BMFS5; TRP53; BCC7
Background

Gene Name: TP53

NCBI Gene Entry: 7157

UniProt Entry: P04637

Application Information

Molecular Weight: Predicted, 44 kDa; observed, 50 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB595

Species Reactivity: Human, mouse, rat

Applications Tested: Western Blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen Recombinant protein of human TP53
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using tumor protein p53 antibody (Cat#6528). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with tumor protein p53 antibody (Cat#6528, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of Hela cells with tumor protein p53 antibody (Cat#6528, 1:1,000). Nuclei were stained blue with DAPI; Tumor protein p53 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of TP53 expression in HeLa cells using TP53 antibody (Cat#6528, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TP53.
Western blotting analysis using tumor protein p53 antibody (Cat#6528). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with tumor protein p53 antibody (Cat#6528, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of Hela cells with tumor protein p53 antibody (Cat#6528, 1:1,000). Nuclei were stained blue with DAPI; Tumor protein p53 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of TP53 expression in HeLa cells using TP53 antibody (Cat#6528, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TP53.
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