Western blotting analysis using DDB1 antibody (Cat#66805). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DDB1 antibody (Cat#66805, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using DDB1 antibody (Cat#66805). DDB1 expression in wild-type (WT) and DDB1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DDB1 antibody (Cat#66805, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
DDB1; Damage Specific DNA Binding Protein 1; Xeroderma Pigmentosum Group E-Complementing Protein; UV-Damaged DNA-Binding Protein 1; UV-Damaged DNA-Binding Factor; DNA Damage-Binding Protein 1; DNA Damage-Binding Protein A; HBV X-Associated Protein 1; XPE-Binding Factor; DDB P127 Subunit; UV-DDB 1; XPE-BF; XAP-1; XAP1; Damage-Specific DNA Binding Protein 1 (127kD); Damage-Specific DNA Binding Protein 1, 127kDa; Damage-Specific DNA-Binding Protein 1; UV-DDB1; WHIKERS; DDBA; XPCE; DDBa; XPCe; XPE
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using DDB1 antibody (Cat#66805). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DDB1 antibody (Cat#66805, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using DDB1 antibody (Cat#66805). DDB1 expression in wild-type (WT) and DDB1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DDB1 antibody (Cat#66805, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using DDB1 antibody (Cat#66805). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DDB1 antibody (Cat#66805, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using DDB1 antibody (Cat#66805). DDB1 expression in wild-type (WT) and DDB1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DDB1 antibody (Cat#66805, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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