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  • Primary Antibody
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Home>Product>Primary Antibody

KD-Validated Integrin β1/CD29 Recombinant Rabbit mAb

#66731

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using Integrin beta-1/CD29 antibody (Cat#66731). Integrin beta-1/CD29 expression in wild type (WT) and Integrin beta-1/CD29 knockout (KO) HeLa cells with 30 μg of Total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Integrin beta-1/CD29 antibody (Cat#66731, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Integrin β1/CD29 antibody (Cat#66731). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Integrin β1/CD29 antibody (Cat#66731,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Integrin beta-1/CD29 antibody (Cat#66731). Integrin beta-1/CD29 expression in wild type (WT) and Integrin beta-1/CD29 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Integrin beta-1/CD29 antibody (Cat#66731,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Integrin beta-1/CD29 expression in HT-1080 cells using Integrin beta-1/CD29 antibody (Cat#66731,1:2,000). Green, isotype control; red, Integrin beta-1/CD29.
Immunocytochemical staining of HT-1080 cells with Integrin beta-1/CD29 antibody (Cat#66731, 1:1,000). Nuclei were stained blue with DAPI; Integrin beta-1/CD29 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human bladder carcinoma using integrin beta-1/CD29 antibody (Cat#66731, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    ITGB1
  • Catalog：
    66731
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated Integrin β1/CD29 Recombinant Rabbit mAb
Aliases GPIIA; MSK12; CD29; FNRB; MDF2; Integrin, Beta 1 (Fibronectin Receptor, Beta Polypeptide, Antigen CD29 Includes MDF2, MSK12); Glycoprotein IIa; Integrin Beta-1; Very Late Activation Protein, Beta Polypeptide; Fibronectin Receptor Subunit Beta; Integrin VLA-4 Beta Subunit; VLA-4 Subunit Beta; Integrin Beta 1; CD29 Antigen; VLA-BETA; VLAB
Background

Gene Name: ITGB1

NCBI Gene Entry: 3688

UniProt Entry: P05556

Application Information

Molecular Weight: Predicted, 88 kDa, observed, 115/135 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB885

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen A synthesized peptide derived from human integrin beta-1/CD29
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using Integrin beta-1/CD29 antibody (Cat#66731). Integrin beta-1/CD29 expression in wild type (WT) and Integrin beta-1/CD29 knockout (KO) HeLa cells with 30 μg of Total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Integrin beta-1/CD29 antibody (Cat#66731, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using Integrin β1/CD29 antibody (Cat#66731). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Integrin β1/CD29 antibody (Cat#66731,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using Integrin beta-1/CD29 antibody (Cat#66731). Integrin beta-1/CD29 expression in wild type (WT) and Integrin beta-1/CD29 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Integrin beta-1/CD29 antibody (Cat#66731,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of Integrin beta-1/CD29 expression in HT-1080 cells using Integrin beta-1/CD29 antibody (Cat#66731,1:2,000). Green, isotype control; red, Integrin beta-1/CD29.
  • Immunocytochemical staining of HT-1080 cells with Integrin beta-1/CD29 antibody (Cat#66731, 1:1,000). Nuclei were stained blue with DAPI; Integrin beta-1/CD29 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Immunohistochemistry was performed on paraffin-embedded human bladder carcinoma using integrin beta-1/CD29 antibody (Cat#66731, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    ITGB1
  • Catalog：
    66731
  • Application：
    WB, FCM, ICC, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using Integrin beta-1/CD29 antibody (Cat#66731). Integrin beta-1/CD29 expression in wild type (WT) and Integrin beta-1/CD29 knockout (KO) HeLa cells with 30 μg of Total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Integrin beta-1/CD29 antibody (Cat#66731, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Integrin β1/CD29 antibody (Cat#66731). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Integrin β1/CD29 antibody (Cat#66731,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Integrin beta-1/CD29 antibody (Cat#66731). Integrin beta-1/CD29 expression in wild type (WT) and Integrin beta-1/CD29 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Integrin beta-1/CD29 antibody (Cat#66731,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Integrin beta-1/CD29 expression in HT-1080 cells using Integrin beta-1/CD29 antibody (Cat#66731,1:2,000). Green, isotype control; red, Integrin beta-1/CD29.
Immunocytochemical staining of HT-1080 cells with Integrin beta-1/CD29 antibody (Cat#66731, 1:1,000). Nuclei were stained blue with DAPI; Integrin beta-1/CD29 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunohistochemistry was performed on paraffin-embedded human bladder carcinoma using integrin beta-1/CD29 antibody (Cat#66731, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
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    support@genuinbiotech.com

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  1. CONNECT:[ UseTime:0.000384s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000760s ]
  3. SELECT * FROM `goods` WHERE `code` = '66731' LIMIT 1 [ RunTime:0.000475s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000422s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000328s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000377s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 8666 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000352s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000418s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 8666 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000291s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000367s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000232s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.068526s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5041,7052,7336,9973,8270,8826,8010,9231,8461,8925) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000888s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000489s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000202s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000543s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000222s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000309s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000379s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000239s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000219s ]
0.106249s