Western blotting analysis using FLT4 antibody (Cat#6192). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FLT4 antibody (Cat#6192, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with FLT4 antibody (Cat#6192,1:1,000). Nuclei were stained blue with DAPI; FLT4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of FLT4 expression in HAP-1 cells using FLT4 antibody (Cat#6192,1:2,000). Green, isotype control; red, FLT4.
Western blotting analysis using FLT4 antibody (Cat#6192). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FLT4 antibody (Cat#6192, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with FLT4 antibody (Cat#6192,1:1,000). Nuclei were stained blue with DAPI; FLT4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of FLT4 expression in HAP-1 cells using FLT4 antibody (Cat#6192,1:2,000). Green, isotype control; red, FLT4.
Western blotting analysis using FLT4 antibody (Cat#6192). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FLT4 antibody (Cat#6192, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HAP-1 cells with FLT4 antibody (Cat#6192,1:1,000). Nuclei were stained blue with DAPI; FLT4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of FLT4 expression in HAP-1 cells using FLT4 antibody (Cat#6192,1:2,000). Green, isotype control; red, FLT4.
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