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Home>Product>Primary Antibody

Keratin 7 Mouse mAb

#6536

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Western blotting analysis using keratin 7 antibody (Cat#6536). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 7 antibody (Cat#6536, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using keratin 7 antibody (Cat#6536, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of keratin 7 expression in A549 cells using keratin 7 antibody (Cat#6536, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Keratin 7.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    KRT7
  • Catalog：
    6536
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name Keratin 7 Mouse mAb
Aliases KRT7; Keratin 7; CK-7; SCL; K7; Keratin, Type II Cytoskeletal 7; Sarcolectin; K2C7; CK7; Keratin, 55K Type II Cytoskeletal; Type-II Keratin Kb7; Keratin 7, Type II; Cytokeratin 7; Keratin, Simple Epithelial Type I, K7; Type II Mesothelial Keratin K7; Cytokeratin-7; Keratin-7
Background

Gene Name: KRT7

NCBI Gene Entry: 3855

UniProt Entry: P08729

Application Information

Molecular Weight: Predicted, 51 kDa; observed, 51 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9920

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)
Immunogen Recombinant protein of human Keratin 7
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:50-1:100
Note This product is for research use only.
Data
  • Western blotting analysis using keratin 7 antibody (Cat#6536). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 7 antibody (Cat#6536, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using keratin 7 antibody (Cat#6536, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Flow cytometric analysis of keratin 7 expression in A549 cells using keratin 7 antibody (Cat#6536, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Keratin 7.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    KRT7
  • Catalog：
    6536
  • Application：
    WB, FCM, IHC-P
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using keratin 7 antibody (Cat#6536). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 7 antibody (Cat#6536, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human lung adenocarcinoma using keratin 7 antibody (Cat#6536, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of keratin 7 expression in A549 cells using keratin 7 antibody (Cat#6536, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Keratin 7.
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