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Home>Product>Primary Antibody

KD-Validated Optineurin Recombinant Rabbit mAb

#65653

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using optineurin antibody (Cat#65653). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with optineurin antibody (Cat#65653, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using optineurin antibody (Cat#65653). Optineurin expression in wild-type (WT) and optineurin (OPTN) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with optineurin antibody (Cat#65653, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using Optineurin antibody (Cat#65653, 1:1,000), Top panel: wild-type (WT); Bottom panal: Optineurin shRNA knockdown (KD). Nuclei were stained blue with DAPI; Optineurin was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HAP1 cells with optineurin antibody (Cat#65653, 1:1,000). Nuclei were stained blue with DAPI; Optineurin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0. 5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Optineurin expression in HAP-1 cells using Optineurin antibody (Cat#65653, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Optineurin.
Validation of Optineurin knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Optineurin antibody (Cat#65653, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
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  • Gene Symbol：
    OPTN
  • Catalog：
    65653
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated Optineurin Recombinant Rabbit mAb
Aliases OPTN; Optineurin; FIP-2; FIP2; HYPL; HIP7; NRP; TFIIIA-INTP; GLC1E; Transcription Factor IIIA-Interacting Protein; Optic Neuropathy-Inducing Protein; Huntingtin-Interacting Protein 7; Huntingtin-Interacting Protein L; E3-14.7K-Interacting Protein; Huntingtin Yeast Partner L; HIP-7; Tumor Necrosis Factor Alpha-Inducible Cellular Protein Containing Leucine Zipper Domains; Transcrption Factor IIIA-Interacting Protein; Glaucoma 1, Open Angle, E (Adult-Onset); Huntingtin Interacting Protein L; Nemo-Related Protein; NEMO-Related Protein; TFIIIA-IntP; ALS12
Background

Gene Name: OPTN

NCBI Gene Entry: 10133

UniProt Entry: Q96CV9

Application Information

Molecular Weight: Predicted, 66 kDa; observed, 70 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4715

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human Optineurin
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using optineurin antibody (Cat#65653). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with optineurin antibody (Cat#65653, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using optineurin antibody (Cat#65653). Optineurin expression in wild-type (WT) and optineurin (OPTN) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with optineurin antibody (Cat#65653, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HeLa cells using Optineurin antibody (Cat#65653, 1:1,000), Top panel: wild-type (WT); Bottom panal: Optineurin shRNA knockdown (KD). Nuclei were stained blue with DAPI; Optineurin was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
  • Immunocytochemical staining of HAP1 cells with optineurin antibody (Cat#65653, 1:1,000). Nuclei were stained blue with DAPI; Optineurin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0. 5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of Optineurin expression in HAP-1 cells using Optineurin antibody (Cat#65653, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Optineurin.
  • Validation of Optineurin knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Optineurin antibody (Cat#65653, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    OPTN
  • Catalog：
    65653
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using optineurin antibody (Cat#65653). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with optineurin antibody (Cat#65653, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using optineurin antibody (Cat#65653). Optineurin expression in wild-type (WT) and optineurin (OPTN) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with optineurin antibody (Cat#65653, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HeLa cells using Optineurin antibody (Cat#65653, 1:1,000), Top panel: wild-type (WT); Bottom panal: Optineurin shRNA knockdown (KD). Nuclei were stained blue with DAPI; Optineurin was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Immunocytochemical staining of HAP1 cells with optineurin antibody (Cat#65653, 1:1,000). Nuclei were stained blue with DAPI; Optineurin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0. 5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of Optineurin expression in HAP-1 cells using Optineurin antibody (Cat#65653, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, Optineurin.
Validation of Optineurin knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Optineurin antibody (Cat#65653, 1:2,000) and analyzed using BD flow cytometer. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS.
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