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Home>Product>Primary Antibody

ARID1A Mouse mAb

#6579

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Western blotting analysis using ARID1A antibody (Cat#6579). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ARID1A antibody (Cat#6579, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with ARID1A antibody (Cat#6579, 1:1,000). Nuclei were stained blue with DAPI; ARID1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ARID1A expression in HAP-1 cells using ARID1A antibody (Cat#6579, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ARID1A.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ARID1A
  • Catalog：
    6579
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name ARID1A Mouse mAb
Aliases ARID1A; AT-Rich Interaction Domain 1A; BAF250; B120; BAF250a; SMARCF1; C1orf4; P270; SWI/SNF-Related, Matrix-Associated, Actin-Dependent Regulator Of Chromatin Subfamily F Member 1; AT-Rich Interactive Domain-Containing Protein 1A; AT Rich Interactive Domain 1A (SWI-Like); ARID Domain-Containing Protein 1A; SWI/SNF Complex Protein P270; BRG1-Associated Factor 250a; SWI-Like Protein; Osa Homolog 1; C10rf4; HOSA1; OSA1; HELD; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily F, Member 1; AT Rich Interactive Domain 1A (SWI- Like); Chromatin Remodeling Factor P250; BRG1-Associated Factor 250; OSA1 Nuclear Protein; Brain Protein 120; BAF250A; BM029; MRD14; CSS2; ELD
Background

Gene Name: ARID1A

NCBI Gene Entry: 8289

UniProt Entry: O14497

Application Information

Molecular Weight: Predicted, 242 kDa; observed, 270 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9640

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen Recombinant protein of human ARID1A
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ARID1A antibody (Cat#6579). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ARID1A antibody (Cat#6579, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with ARID1A antibody (Cat#6579, 1:1,000). Nuclei were stained blue with DAPI; ARID1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of ARID1A expression in HAP-1 cells using ARID1A antibody (Cat#6579, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ARID1A.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ARID1A
  • Catalog：
    6579
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ARID1A antibody (Cat#6579). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ARID1A antibody (Cat#6579, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with ARID1A antibody (Cat#6579, 1:1,000). Nuclei were stained blue with DAPI; ARID1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ARID1A expression in HAP-1 cells using ARID1A antibody (Cat#6579, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, ARID1A.
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