KO-Validated CDH2 Recombinant Rabbit mAb - Product

KO-Validated CDH2 Recombinant Rabbit mAb

#71176

Click to copy product information

Return to List

Recombinant Antibody
Knockout Validated

Western blotting analysis using CDH2 antibody (Cat#71176). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDH2 antibody (Cat#71176, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Western blotting analysis using CDH2 antibody (Cat#71176). CDH2 expression in wild type (WT) and CDH2 knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CDH2 antibody (Cat#71176, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Flow cytometric analysis of CDH2 expression in HepG2 cells using CDH2 antibody (Cat#71176, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CDH2.

Please select the specifications of the product.

Specification：

Unit price：

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

CDH2
Catalog：

71176
Application：

WB, FCM
Species Reactivity：

H, M

Information

Data

Information

Product Name	KO-Validated CDH2 Recombinant Rabbit mAb
Aliases	CDH2; Cadherin 2; CDHN; CD325; NCAD; Cadherin 2, Type 1, N-Cadherin (Neuronal); Neural Cadherin; N-Cadherin; Cadherin-2; CDw325; Calcium-Dependent Adhesion Protein, Neuronal; CD325 Antigen; N-Cadherin 1; ARVD14; ACOGS; ADHD8
Background	Gene Name: CDH2 NCBI Gene Entry: 1000 UniProt Entry: P19022
Application Information	Molecular Weight: Predicted, 100 kDa; observed, 140 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB2075 Species Reactivity: Human, mouse Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human CDH2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using CDH2 antibody (Cat#71176). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CDH2 antibody (Cat#71176, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using CDH2 antibody (Cat#71176). CDH2 expression in wild type (WT) and CDH2 knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CDH2 antibody (Cat#71176, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of CDH2 expression in HepG2 cells using CDH2 antibody (Cat#71176, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, CDH2.

Please select the specifications of the product.

Specification：

Unit price：

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

CDH2
Catalog：

71176
Application：

WB, FCM
Species Reactivity：

H, M

Datasheet

Home

About

Standards

Product

Custom Services

Signal Pathways

Technical Support

News