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Ribosomal Protein S19 Recombinant Rabbit mAb

#3113

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Recombinant Antibody
Western blotting analysis using ribosomal protein S19 antibody (Cat#3113). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ribosomal protein S19 antibody (Cat#3113, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with ribosomal protein S19 antibody (Cat#3113, 1:1,000). Nuclei were stained blue with DAPI; Ribosomal protein S19 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High.Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ribosomal protein S19 expression in C2C12 cells using ribosomal protein S19 antibody (Cat#3113, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ribosomal protein S19.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    RPS19
  • Catalog:
    3113
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name Ribosomal Protein S19 Recombinant Rabbit mAb
Aliases RPS19; Ribosomal Protein S19; ES19; S19; DBA; Loss Of Heterozygosity On Chromosome 19, Region 1; Loss Of Heterozygosity, 19, Chromosomal Region 1; Small Ribosomal Subunit Protein ES19; 40S Ribosomal Protein S19; LOH19CR1; Diamond-Blackfan Anemia; DBA1
Background

Gene Name: RPS19

NCBI Gene Entry: 6223

UniProt Entry: P39019

Application Information

Molecular Weight: Predicted, 16 kDa; observed, 16 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB4130

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human RPS19
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using ribosomal protein S19 antibody (Cat#3113). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ribosomal protein S19 antibody (Cat#3113, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with ribosomal protein S19 antibody (Cat#3113, 1:1,000). Nuclei were stained blue with DAPI; Ribosomal protein S19 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High.Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
  • Flow cytometric analysis of ribosomal protein S19 expression in C2C12 cells using ribosomal protein S19 antibody (Cat#3113, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ribosomal protein S19.
Western blotting analysis using ribosomal protein S19 antibody (Cat#3113). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ribosomal protein S19 antibody (Cat#3113, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with ribosomal protein S19 antibody (Cat#3113, 1:1,000). Nuclei were stained blue with DAPI; Ribosomal protein S19 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High.Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Flow cytometric analysis of ribosomal protein S19 expression in C2C12 cells using ribosomal protein S19 antibody (Cat#3113, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, ribosomal protein S19.
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