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KD-Validated NME4 Mouse mAb

#65836

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shRNA Knockdown Validated
Western blotting analysis using NME4 antibody (Cat#65836). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NME4 antibody (Cat#65836, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NME4 antibody (Cat#65836). NME4 expression in wild-type (WT) and NME4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NME4 antibody (Cat#65836, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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  • Gene Symbol:
    NME4
  • Catalog:
    65836
  • Application:
    WB
  • Species Reactivity:
    H
Information
Product Name KD-Validated NME4 Mouse mAb
Aliases NME4; NME/NM23 Nucleoside Diphosphate Kinase 4; Nm23-H4; NDPKD; NM23H4; Non-Metastatic Cells 4, Protein Expressed In; Nucleoside Diphosphate Kinase, Mitochondrial; Nucleoside Diphosphate Kinase D; NDP Kinase, Mitochondrial; EC 2.7.4.6; NDK; NDP Kinase D; NDPK-D; NM23D
Background

Gene Name: NME4

NCBI Gene Entry: 4833

UniProt Entry: O00746

Application Information

Molecular Weight: Predicted, 21 kDa; observed, 16 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5105

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen Recombinant protein of human NME4
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Note This product is for research use only.
Data
  • Western blotting analysis using NME4 antibody (Cat#65836). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NME4 antibody (Cat#65836, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using NME4 antibody (Cat#65836). NME4 expression in wild-type (WT) and NME4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NME4 antibody (Cat#65836, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NME4 antibody (Cat#65836). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NME4 antibody (Cat#65836, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NME4 antibody (Cat#65836). NME4 expression in wild-type (WT) and NME4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NME4 antibody (Cat#65836, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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