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Home>Product>Primary Antibody

KD-Validated TBL1XR1 Mouse mAb

#65932

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shRNA Knockdown Validated
Western blotting analysis using TBL1XR1 antibody (Cat#65932). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TBL1XR1 antibody (Cat#65932, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using TBL1XR1 antibody (Cat#65932). TBL1XR1 expression in wild-type (WT) and TBL1XR1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TBL1XR1 antibody (Cat#65932, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of TBL1XR1 expression in HepG2 cells using TBL1XR1 antibody (Cat#65932, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TBL1XR1.
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  • Gene Symbol：
    TBL1XR1
  • Catalog：
    65932
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated TBL1XR1 Mouse mAb
Aliases TBL1XR1; TBL1X/Y Related 1; TBLR1; IRA1; F-Box-Like/WD Repeat-Containing Protein TBL1XR1; DC42; C21; Nuclear Receptor Corepressor/HDAC3 Complex Subunit TBLR1; Transducin Beta Like 1 X-Linked Receptor 1; TBL1-Related Protein 1; TBL1X Receptor 1; FLJ12894; Nuclear Receptor Co-Repressor/HDAC3 Complex Subunit; Transducin (Beta)-Like 1X-Linked Receptor 1; Transducin Beta-Like 1X-Related Protein 1; TBL1-Related Protein; MRD41
Background

Gene Name: TBL1XR1

NCBI Gene Entry: 79718

UniProt Entry: Q9BZK7

Application Information

Molecular Weight: Predicted, 56 kDa; observed, 49-56 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB5895

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human TBL1XR1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using TBL1XR1 antibody (Cat#65932). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TBL1XR1 antibody (Cat#65932, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using TBL1XR1 antibody (Cat#65932). TBL1XR1 expression in wild-type (WT) and TBL1XR1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TBL1XR1 antibody (Cat#65932, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of TBL1XR1 expression in HepG2 cells using TBL1XR1 antibody (Cat#65932, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TBL1XR1.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    TBL1XR1
  • Catalog：
    65932
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using TBL1XR1 antibody (Cat#65932). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TBL1XR1 antibody (Cat#65932, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using TBL1XR1 antibody (Cat#65932). TBL1XR1 expression in wild-type (WT) and TBL1XR1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TBL1XR1 antibody (Cat#65932, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of TBL1XR1 expression in HepG2 cells using TBL1XR1 antibody (Cat#65932, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, TBL1XR1.
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