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Home>Product>Primary Antibody

KD-Validated UCHL1 Rabbit pAb

#66106

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shRNA Knockdown Validated
Western blotting analysis using UCHL1 antibody (Cat#66106). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UCHL1 antibody (Cat#66106, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using UCHL1 antibody (Cat#66106). UCHL1 expression in wild-type (WT) and UCHL1 shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with UCHL1 antibody (Cat#66106, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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Specification：
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  • Gene Symbol：
    UCHL1
  • Catalog：
    66106
  • Application：
    WB
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated UCHL1 Rabbit pAb
Aliases UCHL1; Ubiquitin C-Terminal Hydrolase L1; PGP9.5; UCHL-1; Ubiquitin Carboxyl-Terminal Esterase L1 (Ubiquitin Thiolesterase); Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1; Neuron Cytoplasmic Protein 9.5; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase; PGP 9.5; Uch-L1; UCH-L1; PARK5; Epididymis Secretory Protein Li 53; Epididymis Luminal Protein 117; EC 3.4.19.12; HEL-S-53; HEL-117; SPG79A; NDGOA; PGP95; SPG79
Background

Gene Name: UCHL1

NCBI Gene Entry: 7345

UniProt Entry: P09936

Application Information

Molecular Weight: Predicted, 25 kDa; observed, 25 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human UCHL1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using UCHL1 antibody (Cat#66106). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UCHL1 antibody (Cat#66106, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using UCHL1 antibody (Cat#66106). UCHL1 expression in wild-type (WT) and UCHL1 shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with UCHL1 antibody (Cat#66106, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    UCHL1
  • Catalog：
    66106
  • Application：
    WB
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using UCHL1 antibody (Cat#66106). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UCHL1 antibody (Cat#66106, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using UCHL1 antibody (Cat#66106). UCHL1 expression in wild-type (WT) and UCHL1 shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with UCHL1 antibody (Cat#66106, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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