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PRKAR2A Recombinant Rabbit mAb

#4646

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Recombinant Antibody
Western blotting analysis using PRKAR2A antibody (Cat#4646). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PRKAR2A antibody (Cat#4646, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using PRKAR2A antibody (Cat#4646, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of PRKAR2A expression in HAP-1 cells using PRKAR2A antibody (Cat#4646, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PRKAR2A.
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Unit price:
$69.00
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1
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  • Gene Symbol:
    PRKAR2A
  • Catalog:
    4646
  • Application:
    WB, FCM, IHC-P
  • Species Reactivity:
    H, R
Information
Product Name PRKAR2A Recombinant Rabbit mAb
Aliases Protein Kinase CAMP-Dependent Type II Regulatory Subunit Alpha; PRKAR2; Protein Kinase, CAMP-Dependent, Regulatory Subunit Type II Alpha; CAMP-Dependent Protein Kinase Type II-Alpha Regulatory Subunit; PKR2; Protein Kinase, CAMP-Dependent, Regulatory, Type II, Alpha; CAMP-Dependent Protein Kinase Regulatory Subunit RII Alpha; Protein Kinase A, RII-Alpha Subunit
Background

Gene Name: PRKAR2A

NCBI Gene Entry: 5576

UniProt Entry: P13861

Application Information

Molecular Weight: Predicted, 46 kDa; observed, 52 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB12240

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunohistochemistry-paraffin (IHC-P)

Immunogen A synthesized peptide derived from human PKA R2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using PRKAR2A antibody (Cat#4646). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PRKAR2A antibody (Cat#4646, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using PRKAR2A antibody (Cat#4646, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Flow cytometric analysis of PRKAR2A expression in HAP-1 cells using PRKAR2A antibody (Cat#4646, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PRKAR2A.
Western blotting analysis using PRKAR2A antibody (Cat#4646). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PRKAR2A antibody (Cat#4646, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using PRKAR2A antibody (Cat#4646, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Flow cytometric analysis of PRKAR2A expression in HAP-1 cells using PRKAR2A antibody (Cat#4646, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, PRKAR2A.
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