Western blotting analysis using suppressor of cytokine signaling 2 antibody (Cat#3356). Total cell lysates (10 μg for HAP1 and 30 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with suppressor of cytokine signaling 2 antibody (Cat#3356, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Suppressor of cytokine signaling 2 expression in HAP-1 cells using Suppressor of cytokine signaling 2 antibody (Cat#3356, 1:2,000). Green, isotype control; red, Suppressor of cytokine signaling 2.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human SOCS2
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using suppressor of cytokine signaling 2 antibody (Cat#3356). Total cell lysates (10 μg for HAP1 and 30 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with suppressor of cytokine signaling 2 antibody (Cat#3356, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Suppressor of cytokine signaling 2 expression in HAP-1 cells using Suppressor of cytokine signaling 2 antibody (Cat#3356, 1:2,000). Green, isotype control; red, Suppressor of cytokine signaling 2.
Western blotting analysis using suppressor of cytokine signaling 2 antibody (Cat#3356). Total cell lysates (10 μg for HAP1 and 30 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with suppressor of cytokine signaling 2 antibody (Cat#3356, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Suppressor of cytokine signaling 2 expression in HAP-1 cells using Suppressor of cytokine signaling 2 antibody (Cat#3356, 1:2,000). Green, isotype control; red, Suppressor of cytokine signaling 2.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000946s ]
SELECT * FROM `goods` WHERE `code` = '3356' LIMIT 1 [ RunTime:0.000909s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000716s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000467s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000544s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 4992 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002941s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000546s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 4992 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000304s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.002214s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000269s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.186768s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5921,9866,7570,8224,9663,10207,9637,5293,9018,9010) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001018s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000642s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.002137s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000807s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000399s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002344s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000682s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000432s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000403s ]
