KD-Validated PLIN3 Mouse mAb - Product - Hefei GenuIN Biotech Co., Ltd.

KD-Validated PLIN3 Mouse mAb

#66108

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shRNA Knockdown Validated

Western blotting analysis using PLIN3 antibody (Cat#66108). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PLIN3 antibody (Cat#66108, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using PLIN3 antibody (Cat#66108). PLIN3 expression in wild-type (WT) and PLIN3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PLIN3 antibody (Cat#66108, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Flow cytometric analysis of PLIN3 expression in HepG2 cells using PLIN3 antibody (Cat#66108, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PLIN3.

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Unit price：

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(Excluding sales tax)

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Gene Symbol：

PLIN3
Catalog：

66108
Application：

WB, FCM
Species Reactivity：

H

Information

Data

Information

Product Name	KD-Validated PLIN3 Mouse mAb
Aliases	PLIN3; Perilipin 3; TIP47; PP17; Placental Protein 17; M6PRBP1; Mannose-6-Phosphate Receptor-Binding Protein 1; Cargo Selection Protein TIP47; 47 KDa MPR-Binding Protein; Perilipin-3; Cargo Selection Protein (Mannose 6 Phosphate Receptor Binding Protein); 47 KDa Mannose 6-Phosphate Receptor-Binding Protein; Mannose-6-Phosphate Receptor Binding Protein 1; Testicular Tissue Protein Li 114; Tail-Interacting Protein, 47 KD; MPR-BINDING PROTEIN, 47-KD
Background	Gene Name: PLIN3 NCBI Gene Entry: 10226 UniProt Entry: O60664
Application Information	Molecular Weight: Predicted, 47 kDa; observed, 47 kDa Clonality: Mouse monoclonal antibody Clone ID: 25GB10105 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human PLIN3
Isotype	Mouse IgG2a kappa
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using PLIN3 antibody (Cat#66108). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PLIN3 antibody (Cat#66108, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PLIN3 antibody (Cat#66108). PLIN3 expression in wild-type (WT) and PLIN3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PLIN3 antibody (Cat#66108, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of PLIN3 expression in HepG2 cells using PLIN3 antibody (Cat#66108, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, PLIN3.

Please select the specifications of the product.

Specification：

Unit price：

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

PLIN3
Catalog：

66108
Application：

WB, FCM
Species Reactivity：

H

Datasheet

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