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WASP Family Member 2 Mouse mAb

#6623

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Western blotting analysis using WASF2 antibody (Cat#6623). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WASF2 antibody (Cat#6623, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using WASF2 antibody (Cat#6623, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Please select the specifications of the product.
Specification:
Unit price:
$69.00
Quantity:
1
(Excluding sales tax)
  • Gene Symbol:
    WASF2
  • Catalog:
    6623
  • Application:
    WB, IHC-P
  • Species Reactivity:
    H
Information
Product Name WASP Family Member 2 Mouse mAb
Aliases WASF2; WASP Family Member 2; WAVE2; SCAR2; Wiskott-Aldrich Syndrome Protein Family Member 2; Verprolin Homology Domain-Containing Protein 2; WASP Family Protein Member 2; WAS Protein Family Member 2; Actin-Binding Protein WASF2; Protein WAVE-2; Putative Wiskott-Aldrich Syndrome Protein Family Member 4; Suppressor Of Cyclic-AMP Receptor (WASP-Family); WASP Family Verprolin-Homologous Protein 2; WAS Protein Family, Member 2; WASP Family Protein Member 4; DJ393P12.2; WASF4; IMD2
Background

Gene Name: WASF2

NCBI Gene Entry: 10163

UniProt Entry: Q9Y6W5

Application Information

Molecular Weight: Predicted, 54 kDa; observed, 80 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9375

Species Reactivity: Human

Applications Tested: Western Blotting (WB), immunohistochemistry-paraffin (IHC-P)

Immunogen Recombinant protein of human WASF2
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using WASF2 antibody (Cat#6623). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WASF2 antibody (Cat#6623, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using WASF2 antibody (Cat#6623, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using WASF2 antibody (Cat#6623). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WASF2 antibody (Cat#6623, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using WASF2 antibody (Cat#6623, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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