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Home>Product>Primary Antibody

KD-Validated NEFL Mouse mAb

#66239

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shRNA Knockdown Validated
Western blotting analysis using neurofilament light chain antibody (Cat#66239). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with neurofilament light chain antibody (Cat#66239, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using neurofilament light chain antibody (Cat#66239). Neurofilament light chain expression in wild-type (WT) and neurofilament light chain (NEFL) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 β serves as a loading control. The blot was incubated with neurofilament light chain antibody (Cat#66239, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using neurofilament light chain antibody (Cat#66239). Neurofilament light chain expression in wild-type (WT) and neurofilament light chain (NEFL) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with neurofilament light chain antibody (Cat#66239, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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  • Gene Symbol：
    NEFL
  • Catalog：
    66239
  • Application：
    WB
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated NEFL Mouse mAb
Aliases NEFL; Neurofilament Light Chain; NF68; NFL; PPP1R110; CMT1F; CMT2E; Protein Phosphatase 1, Regulatory Subunit 110; Neurofilament, Light Polypeptide 68kDa; Neurofilament Light Polypeptide; Neurofilament Triplet L Protein; NF-L; Light Molecular Weight Neurofilament Protein; Neurofilament Protein, Light Chain; Neurofilament, Light Polypeptide; 68 KDa Neurofilament Protein; Neurofilament Subunit NF-L; Neurofilament Light; CMTDIG
Background

Gene Name: NEFL

NCBI Gene Entry: 4747

UniProt Entry: P07196

Application Information

Molecular Weight: Predicted, 62 kDa; observed, 65 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB8135

Species Reactivity: Human

Applications Tested: Western blotting (WB)
Immunogen Recombinant protein of human NEFL
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using neurofilament light chain antibody (Cat#66239). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with neurofilament light chain antibody (Cat#66239, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using neurofilament light chain antibody (Cat#66239). Neurofilament light chain expression in wild-type (WT) and neurofilament light chain (NEFL) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 β serves as a loading control. The blot was incubated with neurofilament light chain antibody (Cat#66239, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using neurofilament light chain antibody (Cat#66239). Neurofilament light chain expression in wild-type (WT) and neurofilament light chain (NEFL) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with neurofilament light chain antibody (Cat#66239, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NEFL
  • Catalog：
    66239
  • Application：
    WB
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using neurofilament light chain antibody (Cat#66239). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with neurofilament light chain antibody (Cat#66239, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using neurofilament light chain antibody (Cat#66239). Neurofilament light chain expression in wild-type (WT) and neurofilament light chain (NEFL) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 β serves as a loading control. The blot was incubated with neurofilament light chain antibody (Cat#66239, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using neurofilament light chain antibody (Cat#66239). Neurofilament light chain expression in wild-type (WT) and neurofilament light chain (NEFL) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with neurofilament light chain antibody (Cat#66239, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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