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Home>Product>Primary Antibody

Epithelial Cell Adhesion Molecule Mouse mAb

#6630

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Western blotting analysis using epithelial cell adhesion molecule antibody (Cat#6630). Total cell lysates (30 μg) from various cell lines and mouse kidney tissue were loaded and separated by SDS-PAGE. The blot was incubated with epithelial cell adhesion molecule antibody (Cat#6630, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using epithelial cell adhesion molecule antibody (Cat#6630, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HACAT cells with epithelial cell adhesion molecule antibody (Cat#6630, 1:1,000). Nuclei were stained blue with DAPI; epithelial cell adhesion molecule was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    EPCAM
  • Catalog：
    6630
  • Application：
    WB, ICC, IHC-P
  • Species Reactivity：
    H

Information

Data

Information
Product Name Epithelial Cell Adhesion Molecule Mouse mAb
Aliases EPCAM; Epithelial Cell Adhesion Molecule; TROP1; KSA; Tumor-Associated Calcium Signal Transducer; GA733-2; TACSTD1; MOC-31; EGP40; EGP-2; KS1/4; MIC18; MK-1; M4S1; ESA; Major Gastrointestinal Tumor-Associated Protein GA733-2; Trophoblast Cell Surface Antigen 1; Adenocarcinoma-Associated Antigen; Cell Surface Glycoprotein Trop-1; Epithelial Glycoprotein 314; TACST-1; Ber-Ep4; Ep-CAM; HEA125; 323/A3; CO-17A; BerEp4; EGP314; EGP34; CD326; MOC31; 17-1A; MH99; Membrane Component, Chromosome 4, Surface Marker (35kD Glycoprotein); Antigen Identified By Monoclonal Antibody AUA1; Human Epithelial Glycoprotein-2; Epithelial Cell Surface Antigen; Epithelial Glycoprotein; KS 1/4 Antigen; CD326 Antigen; BER-EP; HEGP314; HNPCC8; LYNCH; BEREP4 5EP-CAM; DIAR5; Ly74; LY74 5; M1S2 4; EGP
Background

Gene Name: EPCAM

NCBI Gene Entry: 4072

UniProt Entry: P16422

Application Information

Molecular Weight: Predicted, 35 kDa; observed, 35 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB10095

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (ICC), immunohistochemistry-paraffin (IHC-P)
Immunogen Recombinant protein of human EPCAM
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note This product is for research use only.
Data
  • Western blotting analysis using epithelial cell adhesion molecule antibody (Cat#6630). Total cell lysates (30 μg) from various cell lines and mouse kidney tissue were loaded and separated by SDS-PAGE. The blot was incubated with epithelial cell adhesion molecule antibody (Cat#6630, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using epithelial cell adhesion molecule antibody (Cat#6630, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunocytochemical staining of HACAT cells with epithelial cell adhesion molecule antibody (Cat#6630, 1:1,000). Nuclei were stained blue with DAPI; epithelial cell adhesion molecule was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$69.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    EPCAM
  • Catalog：
    6630
  • Application：
    WB, ICC, IHC-P
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using epithelial cell adhesion molecule antibody (Cat#6630). Total cell lysates (30 μg) from various cell lines and mouse kidney tissue were loaded and separated by SDS-PAGE. The blot was incubated with epithelial cell adhesion molecule antibody (Cat#6630, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using epithelial cell adhesion molecule antibody (Cat#6630, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HACAT cells with epithelial cell adhesion molecule antibody (Cat#6630, 1:1,000). Nuclei were stained blue with DAPI; epithelial cell adhesion molecule was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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