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Home>Product>Primary Antibody

KD-Validated DFFA Mouse mAb

#66484

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shRNA Knockdown Validated
Western blotting analysis using DFFA antibody (Cat#66484). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DFFA antibody (Cat#66484, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using DFFA antibody (Cat#66484).DFFA expression in wild-type (WT) and DFFA shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DFFA antibody (Cat#66484, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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  • Gene Symbol：
    DFFA
  • Catalog：
    66484
  • Application：
    WB
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated DFFA Mouse mAb
Aliases DFFA; DNA Fragmentation Factor Subunit Alpha; DFF-45; DFF45; ICAD; DFF1; DNA Fragmentation Factor, 45kDa, Alpha Polypeptide; DNA Fragmentation Factor 45 KDa Subunit; Inhibitor Of CAD; DNA Fragmentation Factor, 45 KD, Alpha Polypeptide; DNA Fragmentation Factor, 45 KD, Alpha Subunit
Background

Gene Name: DFFA

NCBI Gene Entry: 1676

UniProt Entry: O00273

Application Information

Molecular Weight: Predicted, 37 kDa; observed, 35, 40 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9530

Species Reactivity: Human

Applications Tested: Western blotting (WB)
Immunogen Recombinant protein of human DFFA
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using DFFA antibody (Cat#66484). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DFFA antibody (Cat#66484, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using DFFA antibody (Cat#66484).DFFA expression in wild-type (WT) and DFFA shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DFFA antibody (Cat#66484, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    DFFA
  • Catalog：
    66484
  • Application：
    WB
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using DFFA antibody (Cat#66484). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DFFA antibody (Cat#66484, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using DFFA antibody (Cat#66484).DFFA expression in wild-type (WT) and DFFA shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DFFA antibody (Cat#66484, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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