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Home>Product>Primary Antibody

KD-Validated ABCB1 Mouse mAb

#66490

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shRNA Knockdown Validated
Western blotting analysis using ABCB1 antibody (Cat#66490). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABCB1 antibody (Cat#66490, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ABCB1 antibody (Cat#66490). ABCB1 expression in wild-type (WT) and ABCB1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ABCB1 antibody (Cat#66490, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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  • Gene Symbol：
    ABCB1
  • Catalog：
    66490
  • Application：
    WB
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated ABCB1 Mouse mAb
Aliases ABCB1; ATP Binding Cassette Subfamily B Member 1; PGY1; Multidrug Resistance Protein 1; CD243; GP170; ABC20; P-170; P-Gp; MDR1; ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1; ATP-Dependent Translocase ABCB1; Phospholipid Transporter ABCB1; Colchicin Sensitivity; P-Glycoprotein 1; CLCS; ATP-Binding Cassette Sub-Family B Member 1; Doxorubicin Resistance; P-Glycoprotein; CD243 Antigen; EC 3.6.3.44; EC 7.6.2.2; EC 7.6.2.1; EC 3.6.3; ENPAT
Background

Gene Name: ABCB1

NCBI Gene Entry: 5243

UniProt Entry: P08183

Application Information

Molecular Weight: Predicted, 141 kDa; observed, 141-250 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9560

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)
Immunogen Recombinant protein of human ABCB1
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using ABCB1 antibody (Cat#66490). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABCB1 antibody (Cat#66490, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using ABCB1 antibody (Cat#66490). ABCB1 expression in wild-type (WT) and ABCB1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ABCB1 antibody (Cat#66490, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    ABCB1
  • Catalog：
    66490
  • Application：
    WB
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using ABCB1 antibody (Cat#66490). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ABCB1 antibody (Cat#66490, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ABCB1 antibody (Cat#66490). ABCB1 expression in wild-type (WT) and ABCB1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ABCB1 antibody (Cat#66490, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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