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Home>Product>Primary Antibody

KD-Validated LGALS3 Mouse mAb

#66844

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shRNA Knockdown Validated
Western blotting analysis using LGALS3 antibody (Cat#66844). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LGALS3 antibody (Cat#66844, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using LGALS3 antibody (Cat#66844). LGALS3 expression in wild type (WT) and LGALS3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with LGALS3 antibody (Cat#66844, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HACAT cells with LGALS3 antibody (Cat#66844, 1:1,000). Nuclei were stained blue with DAPI; LGALS3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
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  • Gene Symbol：
    LGALS3
  • Catalog：
    66844
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M

Information

Data

Information
Product Name KD-Validated LGALS3 Mouse mAb
Aliases LGALS3; Galectin 3; GALIG; Advanced Glycation End-Product Receptor 3; Lectin, Galactoside-Binding, Soluble, 3; Carbohydrate-Binding Protein 35; Galactose-Specific Lectin 3; Laminin-Binding Protein; IgE-Binding Protein; 35 KDa Lectin; Lectin L-29; Galectin-3; LGALS2; MAC-2; GALBP; MAC2; Epididymis Secretory Sperm Binding Protein; Galactoside-Binding Protein; MAC-2 Antigen; Mac-2 Antigen; CBP 35; CBP35; Gal-3; GAL3; L-31; L31
Background

Gene Name: LGALS3

NCBI Gene Entry: 3958

UniProt Entry: P17931

Application Information

Molecular Weight: Predicted, 26 kDa; observed, 30 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 26GB1975

Species Reactivity: Human, mouse

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human LGALS3
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using LGALS3 antibody (Cat#66844). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LGALS3 antibody (Cat#66844, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using LGALS3 antibody (Cat#66844). LGALS3 expression in wild type (WT) and LGALS3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with LGALS3 antibody (Cat#66844, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HACAT cells with LGALS3 antibody (Cat#66844, 1:1,000). Nuclei were stained blue with DAPI; LGALS3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    LGALS3
  • Catalog：
    66844
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M
RELATED PRODUCTS
Western blotting analysis using LGALS3 antibody (Cat#66844). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LGALS3 antibody (Cat#66844, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using LGALS3 antibody (Cat#66844). LGALS3 expression in wild type (WT) and LGALS3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with LGALS3 antibody (Cat#66844, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HACAT cells with LGALS3 antibody (Cat#66844, 1:1,000). Nuclei were stained blue with DAPI; LGALS3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar, 20 μm.
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