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Home>Product>Primary Antibody

KD-Validated OTUB1 Recombinant Rabbit mAb

#66913

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using OTUB1 antibody (Cat#66913). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with OTUB1 antibody (Cat#66913, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using OTUB1 antibody (Cat#66913). OTUB1 expression in wild type (WT) and OTUB1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with OTUB1 antibody (Cat#66913, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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Unit price：
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  • Gene Symbol：
    OTUB1
  • Catalog：
    66913
  • Application：
    WB
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated OTUB1 Recombinant Rabbit mAb
Aliases OTUB1; OTU Deubiquitinase, Ubiquitin Aldehyde Binding 1; OTU Domain-Containing Ubiquitin Aldehyde-Binding Protein 1; Ubiquitin-Specific-Processing Protease OTUB1; OTU Domain, Ubiquitin Aldehyde Binding 1; Deubiquitinating Enzyme OTUB1; Ubiquitin Thioesterase OTUB1; Otubain-1; FLJ20113; FLJ40710; OTB1; OTU1; Ubiquitin-Specific Protease Otubain 1; OTU-Domain Ubal-Binding 1; EC 3.4.19.12; EC 3.1.2; HSPC263; HOTU1
Background

Gene Name: OTUB1

NCBI Gene Entry: 55611

UniProt Entry: Q96FW1

Application Information

Molecular Weight: Predicted, 31 kDa; observed, 33 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB1310

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB)
Immunogen A synthesized peptide derived from human OTUB1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Data
  • Western blotting analysis using OTUB1 antibody (Cat#66913). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with OTUB1 antibody (Cat#66913, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using OTUB1 antibody (Cat#66913). OTUB1 expression in wild type (WT) and OTUB1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with OTUB1 antibody (Cat#66913, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    OTUB1
  • Catalog：
    66913
  • Application：
    WB
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using OTUB1 antibody (Cat#66913). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with OTUB1 antibody (Cat#66913, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using OTUB1 antibody (Cat#66913). OTUB1 expression in wild type (WT) and OTUB1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with OTUB1 antibody (Cat#66913, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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