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Home>Product>Primary Antibody

KD-Validated YWHAZ Recombinant mAb

#66977

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using YWHAZ antibody (Cat#66977). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with YWHAZ antibody (Cat#66977, 1:5,000) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:10,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using YWHAZ antibody (Cat#66977). YWHAZ expression in wild-type (WT) and YWHAZ shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with YWHAZ antibody (Cat#66977, 1:5,000) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:10,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with YWHAZ antibody (Cat#66977, 1:1,000). Nuclei were stained blue with DAPI; YWHAZ was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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  • Gene Symbol：
    YWHAZ
  • Catalog：
    66977
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated YWHAZ Recombinant mAb
Aliases YWHAZ; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Zeta; KCIP-1; 14-3-3-Zeta; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Delta Polypeptide; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Zeta Polypeptide; 14-3-3 Protein Zeta/Delta; 14-3-3 Delta; 14-3-3 Zeta; YWHAD; Tyrosine 3/Tryptophan 5 -Monooxygenase Activation Protein, Zeta Polypeptide; 14-3-3 Protein/Cytosolic Phospholipase A2; Protein Kinase C Inhibitor Protein-1; Protein Kinase C Inhibitor Protein 1; Epididymis Secretory Protein Li 93; Epididymis Secretory Protein Li 3; Epididymis Luminal Protein 4; Phospholipase A2; HEL-S-93; HEL-S-3; POPCHAS; HEL4
Background

Gene Name: YWHAZ

NCBI Gene Entry: 7534

UniProt Entry: P63104

Application Information

Molecular Weight: Predicted, 28 kDa; observed, 28 kDa

Clonality: Human monoclonal antibody

Clone ID: 26GB2585

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human YWHAZ
Isotype Human IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.03% Proclin 300.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using YWHAZ antibody (Cat#66977). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with YWHAZ antibody (Cat#66977, 1:5,000) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:10,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using YWHAZ antibody (Cat#66977). YWHAZ expression in wild-type (WT) and YWHAZ shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with YWHAZ antibody (Cat#66977, 1:5,000) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:10,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HT-1080 cells with YWHAZ antibody (Cat#66977, 1:1,000). Nuclei were stained blue with DAPI; YWHAZ was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    YWHAZ
  • Catalog：
    66977
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using YWHAZ antibody (Cat#66977). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with YWHAZ antibody (Cat#66977, 1:5,000) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:10,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using YWHAZ antibody (Cat#66977). YWHAZ expression in wild-type (WT) and YWHAZ shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with YWHAZ antibody (Cat#66977, 1:5,000) and HRP-conjugated goat anti-human secondary antibody (Cat#401, 1:10,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HT-1080 cells with YWHAZ antibody (Cat#66977, 1:1,000). Nuclei were stained blue with DAPI; YWHAZ was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Scale bar: 20 μm.
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