Western blotting analysis using BNlP3L antibody (Cat#67009). Total cell lysates (40 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BNlP3L antibody (Cat#67009, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:5,000) respectively. Image was developed using PiQ™ ECL Substrate Kit (Cat#636).
Western blotting analysis using BNlP3L antibody (Cat#67009). Total lysates (35 μg) from various mouse tissues were loaded and separated by SDS-PAGE. The blot was incubated with BNlP3L antibody (Cat#67009, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively. Image was developed using PiQ™ ECL Substrate Kit (Cat#636).
Immunocytochemical staining of HeLa cells using BNIP3L antibody (Cat#67009, 1:100). Top panel: wild type (WT); Middle panel: wild type (WT) treated with 100 μM cobalt chloride (CoCl2) overnight; Bottom panel: BNIP3L shRNA knockdown (KD) treated with 100 μM cobalt chloride (CoCl2) overnight. Nuclei were stained blue with DAPI. Actin was stained green with Alexa Fluor 488. BNIP3L was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen
A synthesized peptide derived from human BNIP3L
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000 Immunocytochemistry (ICC): 1:100
Note
This product is for research use only.
Data
Western blotting analysis using BNlP3L antibody (Cat#67009). Total cell lysates (40 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BNlP3L antibody (Cat#67009, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:5,000) respectively. Image was developed using PiQ™ ECL Substrate Kit (Cat#636).
Western blotting analysis using BNlP3L antibody (Cat#67009). Total lysates (35 μg) from various mouse tissues were loaded and separated by SDS-PAGE. The blot was incubated with BNlP3L antibody (Cat#67009, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively. Image was developed using PiQ™ ECL Substrate Kit (Cat#636).
Immunocytochemical staining of HeLa cells using BNIP3L antibody (Cat#67009, 1:100). Top panel: wild type (WT); Middle panel: wild type (WT) treated with 100 μM cobalt chloride (CoCl2) overnight; Bottom panel: BNIP3L shRNA knockdown (KD) treated with 100 μM cobalt chloride (CoCl2) overnight. Nuclei were stained blue with DAPI. Actin was stained green with Alexa Fluor 488. BNIP3L was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
Western blotting analysis using BNlP3L antibody (Cat#67009). Total cell lysates (40 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BNlP3L antibody (Cat#67009, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:5,000) respectively. Image was developed using PiQ™ ECL Substrate Kit (Cat#636).
Western blotting analysis using BNlP3L antibody (Cat#67009). Total lysates (35 μg) from various mouse tissues were loaded and separated by SDS-PAGE. The blot was incubated with BNlP3L antibody (Cat#67009, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively. Image was developed using PiQ™ ECL Substrate Kit (Cat#636).
Immunocytochemical staining of HeLa cells using BNIP3L antibody (Cat#67009, 1:100). Top panel: wild type (WT); Middle panel: wild type (WT) treated with 100 μM cobalt chloride (CoCl2) overnight; Bottom panel: BNIP3L shRNA knockdown (KD) treated with 100 μM cobalt chloride (CoCl2) overnight. Nuclei were stained blue with DAPI. Actin was stained green with Alexa Fluor 488. BNIP3L was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
Shopping cart ()
Estimated total price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
