KO-Validated Polybromo 1 Recombinant Rabbit mAb - Product

KO-Validated Polybromo 1 Recombinant Rabbit mAb

#71239

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Recombinant Antibody
Knockout Validated

Western blotting analysis using polybromo 1 antibody (Cat#71239). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with polybromo 1 antibody (Cat#71239, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Western blotting analysis using polybromo 1 antibody (Cat#71239). Polybromo 1 expression in wild-type (WT) and polybromo 1 (PBRM1) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with polybromo 1 antibody (Cat#71239, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Flow cytometric analysis of Polybromo 1 expression in HepG2 cells using Polybromo 1 antibody (Cat#71239, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Polybromo 1.

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Unit price：

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Gene Symbol：

PBRM1
Catalog：

71239
Application：

WB, FCM
Species Reactivity：

H, M, R

Information

Data

Information

Product Name	KO-Validated Polybromo 1 Recombinant Rabbit mAb
Aliases	PBRM1; Polybromo 1; BAF180; PB1; SMARCH1; BRG1-Associated Factor 180; Protein Polybromo-1; Polybromo-1D; HPB1; RCC
Background	Gene Name: PBRM1 NCBI Gene Entry: 55193 UniProt Entry: Q86U86
Application Information	Molecular Weight: Predicted, 193 kDa; observed, 193 kDa Clonality: Rabbit monoclonal antibody Clone ID: 25GB3605 Species Reactivity: Human,mouse,rat Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human PBRM1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note	This product is for research use only.

Data

Western blotting analysis using polybromo 1 antibody (Cat#71239). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with polybromo 1 antibody (Cat#71239, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using polybromo 1 antibody (Cat#71239). Polybromo 1 expression in wild-type (WT) and polybromo 1 (PBRM1) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with polybromo 1 antibody (Cat#71239, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Polybromo 1 expression in HepG2 cells using Polybromo 1 antibody (Cat#71239, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 100% Methanol, chill on -20℃ before use. Green, isotype control; red, Polybromo 1.

Please select the specifications of the product.

Specification：

Unit price：

Quantity：

(Excluding sales tax)

Add to Cart

Gene Symbol：

PBRM1
Catalog：

71239
Application：

WB, FCM
Species Reactivity：

H, M, R

Datasheet

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