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KD-Validated PFKFB2 Recombinant Rabbit mAb

#67026

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using PFKFB2 antibody (Cat#67026, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using PFKFB2 antibody (Cat#67026, 1:1,000). PFKFB2 expression in wild-type (WT) and PFKFB2 shRNA knockdown (KD) MCF7 cells of total cell lysates. GAPDH serves as a loading control.
Immunocytochemical staining of MCF7 cells using PFKFB2 antibody (Cat#67026, 1:200). Top panel: wild type (WT); Bottom panel: PFKFB2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; PFKFB2 was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
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  • Gene Symbol:
    PFKFB2
  • Catalog:
    67026
  • Application:
    WB, ICC
  • Species Reactivity:
    H, Mk
Information
Product Name KD-Validated PFKFB2 Recombinant Rabbit mAb
Aliases PFKFB2; 6-Phosphofructo-2-Kinase/Fructose-2,6-Biphosphatase 2; 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase 2; 6PF-2-K/Fru-2,6-P2ase 2; PFK/FBPase 2; Fructose-2,6-Bisphosphatase, Cardiac Isozyme; 6PF-2-K/Fru-2,6-P2ASE Heart-Type Isozyme; 6PF-2-K/Fru-2,6-P2ase Heart-Type Isozyme; PFK-2/FBPase-2; PFKFB, Cardiac
Background

Gene Name: PFKFB2

NCBI Gene Entry: 5208

UniProt Entry: O60825

Application Information

Molecular Weight: Predicted, 58 kDa; observed, 55 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB2450

Species Reactivity: Human, monkey

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human PFKFB2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000
Immunocytochemistry (ICC): 1:200
Note This product is for research use only.
Data
  • Western blotting analysis using PFKFB2 antibody (Cat#67026, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
  • Western blotting analysis using PFKFB2 antibody (Cat#67026, 1:1,000). PFKFB2 expression in wild-type (WT) and PFKFB2 shRNA knockdown (KD) MCF7 cells of total cell lysates. GAPDH serves as a loading control.
  • Immunocytochemical staining of MCF7 cells using PFKFB2 antibody (Cat#67026, 1:200). Top panel: wild type (WT); Bottom panel: PFKFB2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; PFKFB2 was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
Western blotting analysis using PFKFB2 antibody (Cat#67026, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using PFKFB2 antibody (Cat#67026, 1:1,000). PFKFB2 expression in wild-type (WT) and PFKFB2 shRNA knockdown (KD) MCF7 cells of total cell lysates. GAPDH serves as a loading control.
Immunocytochemical staining of MCF7 cells using PFKFB2 antibody (Cat#67026, 1:200). Top panel: wild type (WT); Bottom panel: PFKFB2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; PFKFB2 was stained magenta with Alexa Fluor 647. Scale bar: 20 μm.
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