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Home>Product>Primary Antibody

KD-Validated PPP2R1A Recombinant Rabbit mAb

#67029

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using PPP2R1A antibody (Cat#67029, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using PPP2R1A antibody (Cat#67029, 1:1,000). PPP2R1A expression in wild-type (WT) and PPP2R1A shRNA knockdown (KD) HeLa cells of total cell lysates. GAPDH serves as a loading control.
Western blotting analysis using PPP2R1A antibody (Cat#67029). Total cell lysates (40 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP2R1A antibody (Cat#67029, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively. Image was developed using PiQ™ ECL Substrate Kit (Cat#636).
Immunocytochemical staining of HeLa cells using PPP2R1A antibody (Cat#67029, 1:100). Nuclei were stained blue with DAPI; β-actin (ACTB) was stained green with Alexa Flour® 488; PPP2R1A was stained magenta with Alexa Flour® 647.
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  • Gene Symbol：
    PPP2R1A
  • Catalog：
    67029
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R, Mk

Information

Data

Information
Product Name KD-Validated PPP2R1A Recombinant Rabbit mAb
Aliases PPP2R1A; Protein Phosphatase 2 Scaffold Subunit Aalpha; Serine/Threonine-Protein Phosphatase 2A 65 KDa Regulatory Subunit A Alpha Isoform; PP2A-Aalpha; PR65A; PP2AA; Protein Phosphatase 2 (Formerly 2A), Regulatory Subunit A (PR 65), Alpha Isoform; Protein Phosphatase 2A, Regulatory Subunit A, Alpha Isoform; Protein Phosphatase 2A Structural Subunit A, Alpha Isoform; Protein Phosphatase 2, Regulatory Subunit A, Alpha; Protein Phosphatase 2, 65kDa Regulatory Subunit A; Medium Tumor Antigen-Associated 61 KDA Protein; Medium Tumor Antigen-Associated 61 KDa Protein; Testicular Secretory Protein Li 1; PP2A Subunit A Isoform PR65-Alpha; PP2A Subunit A Isoform R1-Alpha; PP2AAALPHA; MRD36; PP2Aa; HJS2
Background

Gene Name: PPP2R1A

NCBI Gene Entry: 5518

UniProt Entry: P30153

Application Information

Molecular Weight: Predicted, 65 kDa; observed, 62 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 26GB2465

Species Reactivity: Human, mouse, rat, monkey

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human PPP2R1A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000
Immunocytochemistry (ICC): 1:100
Note This product is for research use only.
Data
  • Western blotting analysis using PPP2R1A antibody (Cat#67029, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
  • Western blotting analysis using PPP2R1A antibody (Cat#67029, 1:1,000). PPP2R1A expression in wild-type (WT) and PPP2R1A shRNA knockdown (KD) HeLa cells of total cell lysates. GAPDH serves as a loading control.
  • Western blotting analysis using PPP2R1A antibody (Cat#67029). Total cell lysates (40 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP2R1A antibody (Cat#67029, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively. Image was developed using PiQ™ ECL Substrate Kit (Cat#636).
  • Immunocytochemical staining of HeLa cells using PPP2R1A antibody (Cat#67029, 1:100). Nuclei were stained blue with DAPI; β-actin (ACTB) was stained green with Alexa Flour® 488; PPP2R1A was stained magenta with Alexa Flour® 647.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    PPP2R1A
  • Catalog：
    67029
  • Application：
    WB, ICC
  • Species Reactivity：
    H, M, R, Mk
RELATED PRODUCTS
Western blotting analysis using PPP2R1A antibody (Cat#67029, 1:1,000). Total cell lysates from various cell lines were loaded and separated by SDS-PAGE.
Western blotting analysis using PPP2R1A antibody (Cat#67029, 1:1,000). PPP2R1A expression in wild-type (WT) and PPP2R1A shRNA knockdown (KD) HeLa cells of total cell lysates. GAPDH serves as a loading control.
Western blotting analysis using PPP2R1A antibody (Cat#67029). Total cell lysates (40 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PPP2R1A antibody (Cat#67029, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:10,000) respectively. Image was developed using PiQ™ ECL Substrate Kit (Cat#636).
Immunocytochemical staining of HeLa cells using PPP2R1A antibody (Cat#67029, 1:100). Nuclei were stained blue with DAPI; β-actin (ACTB) was stained green with Alexa Flour® 488; PPP2R1A was stained magenta with Alexa Flour® 647.
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