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Home>Product>Primary Antibody

KD-Validated APPL1 Mouse mAb

#66489

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shRNA Knockdown Validated
Western blotting analysis using APPL1 antibody (Cat#66489). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with APPL1 antibody (Cat#66489, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using APPL1 antibody (Cat#66489). APPL1 expression in wild-type (WT) and APPL1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with APPL1 antibody (Cat#66489, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of APPL1 expression in HAP-1 cells using APPL1 antibody (Cat#66489, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, APPL1.
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  • Gene Symbol：
    APPL1
  • Catalog：
    66489
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R

Information

Data

Information
Product Name KD-Validated APPL1 Mouse mAb
Aliases APPL1; Adaptor Protein, Phosphotyrosine Interacting With PH Domain And Leucine Zipper 1; APPL; DCC-Interacting Protein 13-Alpha; Adaptor Protein, Phosphotyrosine Interaction, PH Domain And Leucine Zipper Containing 1; Adapter Protein Containing PH Domain, PTB Domain And Leucine Zipper Motif 1; Dip13-Alpha; Adaptor Protein Containing PH Domain, PTB Domain And Leucine Zipper Motif 1; Signaling Adaptor Protein DIP13alpha; AKT2 Interactor; DIP13alpha; KIAA1428; MODY14; DIP13A
Background

Gene Name: APPL1

NCBI Gene Entry: 26060

UniProt Entry: Q9UKG1

Application Information

Molecular Weight: Predicted, 80 kDa; observed, 75 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9555

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen Recombinant protein of human APPL1
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using APPL1 antibody (Cat#66489). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with APPL1 antibody (Cat#66489, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using APPL1 antibody (Cat#66489). APPL1 expression in wild-type (WT) and APPL1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with APPL1 antibody (Cat#66489, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of APPL1 expression in HAP-1 cells using APPL1 antibody (Cat#66489, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, APPL1.
Please select the specifications of the product.
Specification：
Unit price：
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    APPL1
  • Catalog：
    66489
  • Application：
    WB, FCM
  • Species Reactivity：
    H, M, R
RELATED PRODUCTS
Western blotting analysis using APPL1 antibody (Cat#66489). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with APPL1 antibody (Cat#66489, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using APPL1 antibody (Cat#66489). APPL1 expression in wild-type (WT) and APPL1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with APPL1 antibody (Cat#66489, 1:4,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of APPL1 expression in HAP-1 cells using APPL1 antibody (Cat#66489, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, APPL1.
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