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Home>Product>Primary Antibody

KD-Validated NDUFA1 Recombinant Rabbit mAb

#64905

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Recombinant Antibody
Knockdown Validated
Western blotting analysis using NDUFA1 antibody (Cat#64905). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFA1 antibody (Cat#64905, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NDUFA1 antibody (Cat#64905). NDUFA1 expression in wild-type (WT) and NDUFA1 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NDUFA1 antibody (Cat#64905, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of NDUFA1 expression in HepG2 cells using NDUFA1 antibody (Cat#64905, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NDUFA1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NDUFA1
  • Catalog：
    64905
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated NDUFA1 Recombinant Rabbit mAb
Aliases NDUFA1; NADH:Ubiquinone Oxidoreductase Subunit A1; CI-MWFE; MWFE; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 1, 7.5kDa; NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Subunit 1; NADH-Ubiquinone Oxidoreductase MWFE Subunit; NADH:Ubiquinone Oxidoreductase (Complex 1); Complex I MWFE Subunit; Type I Dehydrogenase; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, 1 (7.5kD, MWFE); Complex I-MWFE; MC1DN12; ZNF183
Background

Gene Name: NDUFA1

NCBI Gene Entry: 4694

UniProt Entry: O15239

Application Information

Molecular Weight: Predicted, 8 kDa; observed, 11 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB13685

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human NDUFA1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using NDUFA1 antibody (Cat#64905). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFA1 antibody (Cat#64905, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using NDUFA1 antibody (Cat#64905). NDUFA1 expression in wild-type (WT) and NDUFA1 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NDUFA1 antibody (Cat#64905, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Flow cytometric analysis of NDUFA1 expression in HepG2 cells using NDUFA1 antibody (Cat#64905, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NDUFA1.
Please select the specifications of the product.
Specification：
10 μL 20 μL 100 μL
Unit price：
$89.00
Quantity：
1
(Excluding sales tax)
Add to Cart
  • Gene Symbol：
    NDUFA1
  • Catalog：
    64905
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using NDUFA1 antibody (Cat#64905). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFA1 antibody (Cat#64905, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NDUFA1 antibody (Cat#64905). NDUFA1 expression in wild-type (WT) and NDUFA1 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NDUFA1 antibody (Cat#64905, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of NDUFA1 expression in HepG2 cells using NDUFA1 antibody (Cat#64905, 1:2,000). Fixative solution: 4% Paraformaldehyde in 1x PBS; Permeabilization solution: 0.5% Triton X-100 in 1x PBS. Green, isotype control; red, NDUFA1.
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